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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

TNXB

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TNXB HXBL, TNX, TNXB1, TNXB2, XB
Sequence Length (AA) Molecular Weight (Da)
4242 458220
Protein Name
Tenascin-X
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MMPAQYALTS SLVLLVLLST ARAGPFSSRS NVTLPAPRPP PQPGGHTVGA
60 70 80 90 100
GVGSPSSQLY EHTVEGGEKQ VVFTHRINLP PSTGCGCPPG TEPPVLASEV
110 120 130 140 150
QALRVRLEIL EELVKGLKEQ CTGGCCPASA QAGTGQTDVR TLCSLHGVFD
160 170 180 190 200
LSRCTCSCEP GWGGPTCSDP TDAEIPPSSP PSASGSCPDD CNDQGRCVRG
210 220 230 240 250
RCVCFPGYTG PSCGWPSCPG DCQGRGRCVQ GVCVCRAGFS GPDCSQRSCP
260 270 280 290 300
RGCSQRGRCE GGRCVCDPGY TGDDCGMRSC PRGCSQRGRC ENGRCVCNPG
310 320 330 340 350
YTGEDCGVRS CPRGCSQRGR CKDGRCVCDP GYTGEDCGTR SCPWDCGEGG
360 370 380 390 400
RCVDGRCVCW PGYTGEDCST RTCPRDCRGR GRCEDGECIC DTGYSGDDCG
410 420 430 440 450
VRSCPGDCNQ RGRCEDGRCV CWPGYTGTDC GSRACPRDCR GRGRCENGVC
460 470 480 490 500
VCNAGYSGED CGVRSCPGDC RGRGRCESGR CMCWPGYTGR DCGTRACPGD
510 520 530 540 550
CRGRGRCVDG RCVCNPGFTG EDCGSRRCPG DCRGHGLCED GVCVCDAGYS
560 570 580 590 600
GEDCSTRSCP GGCRGRGQCL DGRCVCEDGY SGEDCGVRQC PNDCSQHGVC
610 620 630 640 650
QDGVCICWEG YVSEDCSIRT CPSNCHGRGR CEEGRCLCDP GYTGPTCATR
660 670 680 690 700
MCPADCRGRG RCVQGVCLCH VGYGGEDCGQ EEPPASACPG GCGPRELCRA
710 720 730 740 750
GQCVCVEGFR GPDCAIQTCP GDCRGRGECH DGSCVCKDGY AGEDCGEEVP
760 770 780 790 800
TIEGMRMHLL EETTVRTEWT PAPGPVDAYE IQFIPTTEGA SPPFTARVPS
810 820 830 840 850
SASAYDQRGL APGQEYQVTV RALRGTSWGL PASKTITTMI DGPQDLRVVA
860 870 880 890 900
VTPTTLELGW LRPQAEVDRF VVSYVSAGNQ RVRLEVPPEA DGTLLTDLMP
910 920 930 940 950
GVEYVVTVTA ERGRAVSYPA SVRANTGSSP LGLLGTTDEP PPSGPSTTQG
960 970 980 990 1000
AQAPLLQQRP QELGELRVLG RDETGRLRVV WTAQPDTFAY FQLRMRVPEG
1010 1020 1030 1040 1050
PGAHEEVLPG DVRQALVPPP PPGTPYELSL HGVPPGGKPS DPIIYQGIMD
1060 1070 1080 1090 1100
KDEEKPGKSS GPPRLGELTV TDRTSDSLLL RWTVPEGEFD SFVIQYKDRD
1110 1120 1130 1140 1150
GQPQVVPVEG PQRSAVITSL DPGRKYKFVL YGFVGKKRHG PLVAEAKILP
1160 1170 1180 1190 1200
QSDPSPGTPP HLGNLWVTDP TPDSLHLSWT VPEGQFDTFM VQYRDRDGRP
1210 1220 1230 1240 1250
QVVPVEGPER SFVVSSLDPD HKYRFTLFGI ANKKRYGPLT ADGTTAPERK
1260 1270 1280 1290 1300
EEPPRPEFLE QPLLGELTVT GVTPDSLRLS WTVAQGPFDS FMVQYKDAQG
1310 1320 1330 1340 1350
QPQAVPVAGD ENEVTVPGLD PDRKYKMNLY GLRGRQRVGP ESVVAKTAPQ
1360 1370 1380 1390 1400
EDVDETPSPT ELGTEAPESP EEPLLGELTV TGSSPDSLSL FWTVPQGSFD
1410 1420 1430 1440 1450
SFTVQYKDRD GRPRAVRVGG KESEVTVGGL EPGHKYKMHL YGLHEGQRVG
1460 1470 1480 1490 1500
PVSAVGVTAP QQEETPPATE SPLEPRLGEL TVTDVTPNSV GLSWTVPEGQ
1510 1520 1530 1540 1550
FDSFIVQYKD KDGQPQVVPV AADQREVTVY NLEPERKYKM NMYGLHDGQR
1560 1570 1580 1590 1600
MGPLSVVIVT APLPPAPATE ASKPPLEPRL GELTVTDITP DSVGLSWTVP
1610 1620 1630 1640 1650
EGEFDSFVVQ YKDRDGQPQV VPVAADQREV TIPDLEPSRK YKFLLFGIQD
1660 1670 1680 1690 1700
GKRRSPVSVE AKTVARGDAS PGAPPRLGEL WVTDPTPDSL RLSWTVPEGQ
1710 1720 1730 1740 1750
FDSFVVQFKD KDGPQVVPVE GHERSVTVTP LDAGRKYRFL LYGLLGKKRH
1760 1770 1780 1790 1800
GPLTADGTTE ARSAMDDTGT KRPPKPRLGE ELQVTTVTQN SVGLSWTVPE
1810 1820 1830 1840 1850
GQFDSFVVQY KDRDGQPQVV PVEGSLREVS VPGLDPAHRY KLLLYGLHHG
1860 1870 1880 1890 1900
KRVGPISAVA ITAGREETET ETTAPTPPAP EPHLGELTVE EATSHTLHLS
1910 1920 1930 1940 1950
WMVTEGEFDS FEIQYTDRDG QLQMVRIGGD RNDITLSGLE SDHRYLVTLY
1960 1970 1980 1990 2000
GFSDGKHVGP VHVEALTVPE EEKPSEPPTA TPEPPIKPRL GELTVTDATP
2010 2020 2030 2040 2050
DSLSLSWTVP EGQFDHFLVQ YRNGDGQPKA VRVPGHEEGV TISGLEPDHK
2060 2070 2080 2090 2100
YKMNLYGFHG GQRMGPVSVV GVTAAEEETP SPTEPSMEAP EPAEEPLLGE
2110 2120 2130 2140 2150
LTVTGSSPDS LSLSWTVPQG RFDSFTVQYK DRDGRPQVVR VGGEESEVTV
2160 2170 2180 2190 2200
GGLEPGRKYK MHLYGLHEGR RVGPVSAVGV TAPEEESPDA PLAKLRLGQM
2210 2220 2230 2240 2250
TVRDITSDSL SLSWTVPEGQ FDHFLVQFKN GDGQPKAVRV PGHEDGVTIS
2260 2270 2280 2290 2300
GLEPDHKYKM NLYGFHGGQR VGPVSAVGLT APGKDEEMAP ASTEPPTPEP
2310 2320 2330 2340 2350
PIKPRLEELT VTDATPDSLS LSWTVPEGQF DHFLVQYKNG DGQPKATRVP
2360 2370 2380 2390 2400
GHEDRVTISG LEPDNKYKMN LYGFHGGQRV GPVSAIGVTA AEEETPSPTE
2410 2420 2430 2440 2450
PSMEAPEPPE EPLLGELTVT GSSPDSLSLS WTVPQGRFDS FTVQYKDRDG
2460 2470 2480 2490 2500
RPQVVRVGGE ESEVTVGGLE PGRKYKMHLY GLHEGRRVGP VSTVGVTAPQ
2510 2520 2530 2540 2550
EDVDETPSPT EPGTEAPGPP EEPLLGELTV TGSSPDSLSL SWTVPQGRFD
2560 2570 2580 2590 2600
SFTVQYKDRD GRPQAVRVGG QESKVTVRGL EPGRKYKMHL YGLHEGRRLG
2610 2620 2630 2640 2650
PVSAVGVTED EAETTQAVPT MTPEPPIKPR LGELTMTDAT PDSLSLSWTV
2660 2670 2680 2690 2700
PEGQFDHFLV QYRNGDGQPK AVRVPGHEDG VTISGLEPDH KYKMNLYGFH
2710 2720 2730 2740 2750
GGQRVGPISV IGVTAAEEET PSPTELSTEA PEPPEEPLLG ELTVTGSSPD
2760 2770 2780 2790 2800
SLSLSWTIPQ GHFDSFTVQY KDRDGRPQVM RVRGEESEVT VGGLEPGRKY
2810 2820 2830 2840 2850
KMHLYGLHEG RRVGPVSTVG VTEDEAETTQ AVPTTTPEPP NKPRLGELTV
2860 2870 2880 2890 2900
TDATPDSLSL SWMVPEGQFD HFLVQYRNGD GQPKVVRVPG HEDGVTISGL
2910 2920 2930 2940 2950
EPDHKYKMNL YGFHGGQRVG PISVIGVTAA EEETPAPTEP STEAPEPPEE
2960 2970 2980 2990 3000
PLLGELTVTG SSPDSLSLSW TIPQGRFDSF TVQYKDRDGR PQVVRVRGEE
3010 3020 3030 3040 3050
SEVTVGGLEP GCKYKMHLYG LHEGQRVGPV SAVGVTAPKD EAETTQAVPT
3060 3070 3080 3090 3100
MTPEPPIKPR LGELTVTDAT PDSLSLSWMV PEGQFDHFLV QYRNGDGQPK
3110 3120 3130 3140 3150
AVRVPGHEDG VTISGLEPDH KYKMNLYGFH GGQRVGPVSA IGVTEEETPS
3160 3170 3180 3190 3200
PTEPSTEAPE APEEPLLGEL TVTGSSPDSL SLSWTVPQGR FDSFTVQYKD
3210 3220 3230 3240 3250
RDGQPQVVRV RGEESEVTVG GLEPGRKYKM HLYGLHEGQR VGPVSTVGIT
3260 3270 3280 3290 3300
APLPTPLPVE PRLGELAVAA VTSDSVGLSW TVAQGPFDSF LVQYRDAQGQ
3310 3320 3330 3340 3350
PQAVPVSGDL RAVAVSGLDP ARKYKFLLFG LQNGKRHGPV PVEARTAPDT
3360 3370 3380 3390 3400
KPSPRLGELT VTDATPDSVG LSWTVPEGEF DSFVVQYKDK DGRLQVVPVA
3410 3420 3430 3440 3450
ANQREVTVQG LEPSRKYRFL LYGLSGRKRL GPISADSTTA PLEKELPPHL
3460 3470 3480 3490 3500
GELTVAEETS SSLRLSWTVA QGPFDSFVVQ YRDTDGQPRA VPVAADQRTV
3510 3520 3530 3540 3550
TVEDLEPGKK YKFLLYGLLG GKRLGPVSAL GMTAPEEDTP APELAPEAPE
3560 3570 3580 3590 3600
PPEEPRLGVL TVTDTTPDSM RLSWSVAQGP FDSFVVQYED TNGQPQALLV
3610 3620 3630 3640 3650
DGDQSKILIS GLEPSTPYRF LLYGLHEGKR LGPLSAEGTT GLAPAGQTSE
3660 3670 3680 3690 3700
ESRPRLSQLS VTDVTTSSLR LNWEAPPGAF DSFLLRFGVP SPSTLEPHPR
3710 3720 3730 3740 3750
PLLQRELMVP GTRHSAVLRD LRSGTLYSLT LYGLRGPHKA DSIQGTARTL
3760 3770 3780 3790 3800
SPVLESPRDL QFSEIRETSA KVNWMPPPSR ADSFKVSYQL ADGGEPQSVQ
3810 3820 3830 3840 3850
VDGQARTQKL QGLIPGARYE VTVVSVRGFE ESEPLTGFLT TVPDGPTQLR
3860 3870 3880 3890 3900
ALNLTEGFAV LHWKPPQNPV DTYDVQVTAP GAPPLQAETP GSAVDYPLHD
3910 3920 3930 3940 3950
LVLHTNYTAT VRGLRGPNLT SPASITFTTG LEAPRDLEAK EVTPRTALLT
3960 3970 3980 3990 4000
WTEPPVRPAG YLLSFHTPGG QNQEILLPGG ITSHQLLGLF PSTSYNARLQ
4010 4020 4030 4040 4050
AMWGQSLLPP VSTSFTTGGL RIPFPRDCGE EMQNGAGASR TSTIFLNGNR
4060 4070 4080 4090 4100
ERPLNVFCDM ETDGGGWLVF QRRMDGQTDF WRDWEDYAHG FGNISGEFWL
4110 4120 4130 4140 4150
GNEALHSLTQ AGDYSMRVDL RAGDEAVFAQ YDSFHVDSAA EYYRLHLEGY
4160 4170 4180 4190 4200
HGTAGDSMSY HSGSVFSARD RDPNSLLISC AVSYRGAWWY RNCHYANLNG
4210 4220 4230 4240 4242
LYGSTVDHQG VSWYHWKGFE FSVPFTEMKL RPRNFRSPAG GG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-2701 Collapse assay details

Data source: Panorama

Official Gene Symbol
TNXB
Peptide Sequence
ILISGLEPSTPYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
3607
Peptide End
3619
CPTAC ID
non-CPTAC-2701
Peptide Molecular Mass
1,444.7926
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 21.2 9.3 12.4 28 17.2 11.4 35.1 19.6 16.8 15 15 15
y11 (2+) 20 8.4 9.6 32.7 8.6 10.9 38.3 12 14.5 15 15 15
b2 (1+) 4.8 6.7 11.6 12.8 7.8 11.2 13.7 10.3 16.1 15 15 15
y6 (1+) 11.5 5.7 9.9 12.9 8 10.7 17.3 9.8 14.6 15 15 15
b4 (1+) 37.7 17.3 25.4 34 16.3 37.2 50.8 23.8 45 15 15 15
sum 6.8 3.8 8.8 11 5.6 9.5 12.9 6.8 12.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments