PROZ, CPTAC-2706 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PROZ	N/A

Sequence Length (AA)	Molecular Weight (Da)
400	44744

Protein Name
Vitamin K-dependent protein Z

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGCVPLLQG	LVLVLALHRV	EPSVFLPASK	ANDVLVRWKR	AGSYLLEELF
60	70	80	90	100
EGNLEKECYE	EICVYEEARE	VFENEVVTDE	FWRRYKGGSP	CISQPCLHNG
110	120	130	140	150
SCQDSIWGYT	CTCSPGYEGS	NCELAKNECH	PERTDGCQHF	CLPGQESYTC
160	170	180	190	200
SCAQGYRLGE	DHKQCVPHDQ	CACGVLTSEK	RAPDLQDLPW	QVKLTNSEGK
210	220	230	240	250
DFCGGVIIRE	NFVLTTAKCS	LLHRNITVKT	YFNRTSQDPL	MIKITHVHVH
260	270	280	290	300
MRYDADAGEN	DLSLLELEWP	IQCPGAGLPV	CTPEKDFAEH	LLIPRTRGLL
310	320	330	340	350
SGWARNGTDL	GNSLTTRPVT	LVEGEECGQV	LNVTVTTRTY	CERSSVAAMH
360	370	380	390	400
WMDGSVVTRE	HRGSWFLTGV	LGSQPVGGQA	HMVLVTKVSR	YSLWFKQIMN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2706 Collapse assay details

Data source: Panorama

Official Gene Symbol: PROZ
Peptide Sequence: GLLSGWAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 298
Peptide End: 305

CPTAC ID: non-CPTAC-2706
Peptide Molecular Mass: 858.4712
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	8.4	6.2	9.7	12.2	7.9	13.9	14.8	10	16.9	15	15	15
y6 (2+)	7	5.2	12.7	17.5	8.9	15.2	18.8	10.3	19.8	15	15	15
b3 (1+)	9.4	4.7	9.8	14.3	8.8	16.2	17.1	10	18.9	15	15	15
y6 (1+)	7.2	7.7	9	10.1	9.7	16.6	12.4	12.4	18.9	15	15	15
y5 (1+)	7.7	6.9	10.6	10.9	9.2	15.6	13.3	11.5	18.9	15	15	15
sum	5	4.9	8.8	10.8	8.5	14.4	11.9	9.8	16.9	15	15	15

PROZ

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-2706 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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PROZ

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-2706 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments