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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

RASGRF1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
RASGRF1 CDC25, GNRP, GRF1
Sequence Length (AA) Molecular Weight (Da)
1273 145234
Protein Name
Ras-specific guanine nucleotide-releasing factor 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQKGIRLNDG HVASLGLLAR KDGTRKGYLS KRSSDNTKWQ TKWFALLQNL
60 70 80 90 100
LFYFESDSSS RPSGLYLLEG CVCDRAPSPK PALSAKEPLE KQHYFTVNFS
110 120 130 140 150
HENQKALELR TEDAKDCDEW VAAIAHASYR TLATEHEALM QKYLHLLQIV
160 170 180 190 200
ETEKTVAKQL RQQIEDGEIE IERLKAEITS LLKDNERIQS TQTVAPNDED
210 220 230 240 250
SDIKKIKKVQ SFLRGWLCRR KWKTIIQDYI RSPHADSMRK RNQVVFSMLE
260 270 280 290 300
AEAEYVQQLH ILVNNFLRPL RMAASSKKPP ITHDDVSSIF LNSETIMFLH
310 320 330 340 350
QIFYQGLKAR ISSWPTLVLA DLFDILLPML NIYQEFVRNH QYSLQILAHC
360 370 380 390 400
KQNRDFDKLL KHYEAKPDCE ERTLETFLTY PMFQIPRYIL TLHELLAHTP
410 420 430 440 450
HEHVERNSLD YAKSKLEELS RIMHDEVSET ENIRKNLAIE RMIIEGCEIL
460 470 480 490 500
LDTSQTFVRQ GSLIQVPMSE KGKITRGRLG SLSLKKEGER QCFLFSKHLI
510 520 530 540 550
ICTRGSGGKL HLTKNGVISL IDCTLLEEPE STEEEAKGSG QDIDHLDFKI
560 570 580 590 600
GVEPKDSPPF TVILVASSRQ EKAAWTSDIS QCVDNIRCNG LMMNAFEENS
610 620 630 640 650
KVTVPQMIKR TREGTREAEM SRSDASLYCD DVDIRFSKTM NSCKVLQIRY
660 670 680 690 700
ASVERLLERL TDLRFLSIDF LNTFLHSYRV FTTAIVVLDK LITIYKKPIS
710 720 730 740 750
AIPARWLRSL ELLFASGQNN KLLYGEPPKS PRATRKFSSP PPLSITKTSS
760 770 780 790 800
PSRRRKLSLN IPIITGGKAL DLAALSCNSN GYTSMYSAMS PFSKATLDTS
810 820 830 840 850
KLYVSSSFTN KIPDEGDTTP EKPEDPSALS KQSSEVSMRE ESDIDQNQSD
860 870 880 890 900
DGDTETSPTK SPTTPKSVKN KNSSEFPLFS YNNGVVMTSC RELDNNRSAL
910 920 930 940 950
SAASAFAIAT AGANEGTPNK EKYRRMSLAS AGFPPDQRNG DKEFVIRRAA
960 970 980 990 1000
TNRVLNVLRH WVSKHSQDFE TNDELKCKVI GFLEEVMHDP ELLTQERKAA
1010 1020 1030 1040 1050
ANIIRTLTQE DPGDNQITLE EITQMAEGVK AEPFENHSAL EIAEQLTLLD
1060 1070 1080 1090 1100
HLVFKKIPYE EFFGQGWMKL EKNERTPYIM KTTKHFNDIS NLIASEIIRN
1110 1120 1130 1140 1150
EDINARVSAI EKWVAVADIC RCLHNYNAVL EITSSMNRSA IFRLKKTWLK
1160 1170 1180 1190 1200
VSKQTKALID KLQKLVSSEG RFKNLREALK NCDPPCVPYL GMYLTDLAFI
1210 1220 1230 1240 1250
EEGTPNYTED GLVNFSKMRM ISHIIREIRQ FQQTAYKIEH QAKVTQYLLD
1260 1270 1273
QSFVMDEESL YESSLRIEPK LPT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5438 Collapse assay details

Data source: Panorama

Official Gene Symbol
RASGRF1
Peptide Sequence
LLYGEPPKSPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
722
Peptide End
732
CPTAC ID
non-CPTAC-5438
Peptide Molecular Mass
1,255.6925
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 22.1 9.8 7.2 26.4 11.4 7 34.4 15 10 15 15 15
y6 (1+) 12.5 7.2 2.9 10.6 8.8 4.2 16.4 11.4 5.1 15 15 15
y8 (1+) 20.5 10.9 7.6 24.3 9.5 9.7 31.8 14.5 12.3 15 15 15
y9 (1+) 19.3 8.5 5.7 29.7 13.7 7.1 35.4 16.1 9.1 15 15 15
sum 10.9 4.9 2.2 12 8.6 4.1 16.2 9.9 4.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-5436 Collapse assay details

Data source: Panorama

Official Gene Symbol
RASGRF1
Peptide Sequence
NSLDYAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
407
Peptide End
413
CPTAC ID
non-CPTAC-5436
Peptide Molecular Mass
809.3919
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.7 2.7 3.1 9.4 3.1 3.5 11 4.1 4.7 15 15 15
y6 (1+) 12.6 4 3.5 15.1 4.8 4.4 19.7 6.2 5.6 15 15 15
y3 (1+) 11.7 3 2.9 14.8 4.9 5.4 18.9 5.7 6.1 15 15 15
y4 (1+) 8.2 2.8 3.3 5.6 4.4 3 9.9 5.2 4.5 15 15 15
sum 4 1.7 1.8 6.3 3.1 2.6 7.5 3.5 3.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-5437 Collapse assay details

Data source: Panorama

Official Gene Symbol
RASGRF1
Peptide Sequence
SLELLFASGQNNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
709
Peptide End
721
CPTAC ID
non-CPTAC-5437
Peptide Molecular Mass
1,419.7358
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 7.4 3.8 3.6 8.1 5 3.6 11 6.3 5.1 15 15 15
y8 (1+) 4.8 5.4 2.9 5.5 5.3 3.6 7.3 7.6 4.6 15 15 15
b4 (1+) 6.1 12 3.6 7.6 14.2 5.4 9.7 18.6 6.5 15 15 15
y5 (1+) 6.9 15.7 7 7.1 23.5 7.3 9.9 28.3 10.1 15 15 15
y6 (1+) 7.7 6.3 1.9 7.7 7.4 3.9 10.9 9.7 4.3 15 15 15
sum 3.4 5.3 1.4 4.5 6.6 2.7 5.6 8.5 3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments