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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

EIF2AK3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
EIF2AK3 PEK, PERK
Sequence Length (AA) Molecular Weight (Da)
1116 125216
Protein Name
Eukaryotic translation initiation factor 2-alpha kinase 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MERAISPGLL VRALLLLLLL LGLAARTVAA GRARGLPAPT AEAAFGLGAA
60 70 80 90 100
AAPTSATRVP AAGAVAAAEV TVEDAEALPA AAGEQEPRGP EPDDETELRP
110 120 130 140 150
RGRSLVIIST LDGRIAALDP ENHGKKQWDL DVGSGSLVSS SLSKPEVFGN
160 170 180 190 200
KMIIPSLDGA LFQWDQDRES METVPFTVES LLESSYKFGD DVVLVGGKSL
210 220 230 240 250
TTYGLSAYSG KVRYICSALG CRQWDSDEME QEEDILLLQR TQKTVRAVGP
260 270 280 290 300
RSGNEKWNFS VGHFELRYIP DMETRAGFIE STFKPNENTE ESKIISDVEE
310 320 330 340 350
QEAAIMDIVI KVSVADWKVM AFSKKGGHLE WEYQFCTPIA SAWLLKDGKV
360 370 380 390 400
IPISLFDDTS YTSNDDVLED EEDIVEAARG ATENSVYLGM YRGQLYLQSS
410 420 430 440 450
VRISEKFPSS PKALESVTNE NAIIPLPTIK WKPLIHSPSR TPVLVGSDEF
460 470 480 490 500
DKCLSNDKFS HEEYSNGALS ILQYPYDNGY YLPYYKRERN KRSTQITVRF
510 520 530 540 550
LDNPHYNKNI RKKDPVLLLH WWKEIVATIL FCIIATTFIV RRLFHPHPHR
560 570 580 590 600
QRKESETQCQ TENKYDSVSG EANDSSWNDI KNSGYISRYL TDFEPIQCLG
610 620 630 640 650
RGGFGVVFEA KNKVDDCNYA IKRIRLPNRE LAREKVMREV KALAKLEHPG
660 670 680 690 700
IVRYFNAWLE APPEKWQEKM DEIWLKDEST DWPLSSPSPM DAPSVKIRRM
710 720 730 740 750
DPFATKEHIE IIAPSPQRSR SFSVGISCDQ TSSSESQFSP LEFSGMDHED
760 770 780 790 800
ISESVDAAYN LQDSCLTDCD VEDGTMDGND EGHSFELCPS EASPYVRSRE
810 820 830 840 850
RTSSSIVFED SGCDNASSKE EPKTNRLHIG NHCANKLTAF KPTSSKSSSE
860 870 880 890 900
ATLSISPPRP TTLSLDLTKN TTEKLQPSSP KVYLYIQMQL CRKENLKDWM
910 920 930 940 950
NGRCTIEERE RSVCLHIFLQ IAEAVEFLHS KGLMHRDLKP SNIFFTMDDV
960 970 980 990 1000
VKVGDFGLVT AMDQDEEEQT VLTPMPAYAR HTGQVGTKLY MSPEQIHGNS
1010 1020 1030 1040 1050
YSHKVDIFSL GLILFELLYP FSTQMERVRT LTDVRNLKFP PLFTQKYPCE
1060 1070 1080 1090 1100
YVMVQDMLSP SPMERPEAIN IIENAVFEDL DFPGKTVLRQ RSRSLSSSGT
1110 1116
KHSRQSNNSH SPLPSN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6086 Collapse assay details

Data source: Panorama

Official Gene Symbol
EIF2AK3
Peptide Sequence
EHIEIIAPSPQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
707
Peptide End
718
CPTAC ID
non-CPTAC-6086
Peptide Molecular Mass
1,388.7412
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 10 5 5.4 10.1 5.6 6.9 14.2 7.5 8.8 16 16 17
b6 (1+) 12.4 6 7.4 15.1 8.9 6.4 19.5 10.7 9.8 16 16 17
b7 (1+) 6.9 9.6 8.3 10.3 10.5 9.6 12.4 14.2 12.7 16 16 17
y5 (1+) 6.7 6.2 5.8 6.4 6.7 4.3 9.3 9.1 7.2 16 16 17
y5 (2+) 7.8 3.8 11.1 9 4.1 10 11.9 5.6 14.9 16 16 17
sum 3.5 2.7 3.7 4.8 3.3 4.2 5.9 4.3 5.6 16 16 17

Stability

Data source: Panorama

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Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
b5 (1+) 7.5 4.9 6.6 1.2 8.5
b6 (1+) 4 11.8 9.3 2.9 4
b7 (1+) 7.6 7.7 4.6 5.2 0.8
y5 (1+) 3 9 7.4 15.8 7.9
y5 (2+) 6.4 6.7 9.7 12.8 7.5
sum 3.4 5 4.2 7.6 6
Fragment ion / Transition all_intra_CV all_inter_CV
b5 (1+) 5.6 6.2
b6 (1+) 7.3 5.9
b7 (1+) 5.6 6.3
y5 (1+) 8.7 8.5
y5 (2+) 8.3 9.5
sum 5.2 5.1

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
b5 (1+) 4.6 139.1 139.2
b6 (1+) 141.3 118.9 184.7
b7 (1+) 28.1 123.6 126.8
y5 (1+) 7.9 25.5 26.7
y5 (2+) 13.1 47.6 49.4
sum 11 25.1 27.4

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments