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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

IGKV4-1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
IGKV4-1 N/A
Sequence Length (AA) Molecular Weight (Da)
121 13380
Protein Name
Immunoglobulin kappa variable 4-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVLQTQVFIS LLLWISGAYG DIVMTQSPDS LAVSLGERAT INCKSSQSVL
60 70 80 90 100
YSSNNKNYLA WYQQKPGQPP KLLIYWASTR ESGVPDRFSG SGSGTDFTLT
110 120 121
ISSLQAEDVA VYYCQQYYST P

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6101 Collapse assay details

Data source: Panorama

Official Gene Symbol
IGKV4-1
Peptide Sequence
LLIYWASTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
72
Peptide End
80
CPTAC ID
non-CPTAC-6101
Peptide Molecular Mass
1,121.6233
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 2.9 2.2 2.8 2.8 5 8 4 5.5 8.5 16 16 17
b3 (1+) 4.1 2.1 3.5 5.4 3.9 3.8 6.8 4.4 5.2 16 16 17
y5 (1+) 5 2 1.1 3 4 8.3 5.8 4.5 8.4 16 16 17
y6 (1+) 5 4.4 3.2 3.5 5.2 8.4 6.1 6.8 9 16 16 17
y7 (1+) 4.2 3.5 4.1 3.7 2.3 5 5.6 4.2 6.5 16 16 17
sum 3.5 1.7 1.4 1.7 2.9 6.4 3.9 3.4 6.6 16 16 17

Stability

Data source: Panorama

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Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
b2 (1+) 8.3 1.7 1.5 2 3.8
b3 (1+) 5 2 0.6 3.3 2.4
y5 (1+) 2.7 1.2 1.6 0.6 2.1
y6 (1+) 3.6 2.8 2.5 5.4 1.7
y7 (1+) 2.8 2.2 2.3 3.4 2.4
sum 2.3 0.6 1 1.7 1
Fragment ion / Transition all_intra_CV all_inter_CV
b2 (1+) 3.2 6
b3 (1+) 2.6 3.1
y5 (1+) 1.6 2
y6 (1+) 3.2 3.8
y7 (1+) 2.6 3
sum 1.2 2.2

Endogenous

Data source: Panorama

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Fragment ion / Transition intra_CV inter_CV total_CV
b2 (1+) 17.1 47.4 50.4
b3 (1+) 30.5 54.5 62.5
y5 (1+) 35.4 39.4 53
y6 (1+) 36.7 83.3 91
y7 (1+) 32.7 62.2 70.3
sum 29.3 38.2 48.1

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments