Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MAP4K4

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP4K4 HGK, KIAA0687, NIK
Sequence Length (AA) Molecular Weight (Da)
1239 142101
Protein Name
Mitogen-activated protein kinase kinase kinase kinase 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MANDSPAKSL VDIDLSSLRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL
60 70 80 90 100
AAIKVMDVTE DEEEEIKLEI NMLKKYSHHR NIATYYGAFI KKSPPGHDDQ
110 120 130 140 150
LWLVMEFCGA GSITDLVKNT KGNTLKEDWI AYISREILRG LAHLHIHHVI
160 170 180 190 200
HRDIKGQNVL LTENAEVKLV DFGVSAQLDR TVGRRNTFIG TPYWMAPEVI
210 220 230 240 250
ACDENPDATY DYRSDLWSCG ITAIEMAEGA PPLCDMHPMR ALFLIPRNPP
260 270 280 290 300
PRLKSKKWSK KFFSFIEGCL VKNYMQRPST EQLLKHPFIR DQPNERQVRI
310 320 330 340 350
QLKDHIDRTR KKRGEKDETE YEYSGSEEEE EEVPEQEGEP SSIVNVPGES
360 370 380 390 400
TLRRDFLRLQ QENKERSEAL RRQQLLQEQQ LREQEEYKRQ LLAERQKRIE
410 420 430 440 450
QQKEQRRRLE EQQRREREAR RQQEREQRRR EQEEKRRLEE LERRRKEEEE
460 470 480 490 500
RRRAEEEKRR VEREQEYIRR QLEEEQRHLE VLQQQLLQEQ AMLLECRWRE
510 520 530 540 550
MEEHRQAERL QRQLQQEQAY LLSLQHDHRR PHPQHSQQPP PPQQERSKPS
560 570 580 590 600
FHAPEPKAHY EPADRAREVE DRFRKTNHSS PEAQSKQTGR VLEPPVPSRS
610 620 630 640 650
ESFSNGNSES VHPALQRPAE PQVPVRTTSR SPVLSRRDSP LQGSGQQNSQ
660 670 680 690 700
AGQRNSTSIE PRLLWERVEK LVPRPGSGSS SGSSNSGSQP GSHPGSQSGS
710 720 730 740 750
GERFRVRSSS KSEGSPSQRL ENAVKKPEDK KEVFRPLKPA DLTALAKELR
760 770 780 790 800
AVEDVRPPHK VTDYSSSSEE SGTTDEEDDD VEQEGADEST SGPEDTRAAS
810 820 830 840 850
SLNLSNGETE SVKTMIVHDD VESEPAMTPS KEGTLIVRQT QSASSTLQKH
860 870 880 890 900
KSSSSFTPFI DPRLLQISPS SGTTVTSVVG FSCDGMRPEA IRQDPTRKGS
910 920 930 940 950
VVNVNPTNTR PQSDTPEIRK YKKRFNSEIL CAALWGVNLL VGTESGLMLL
960 970 980 990 1000
DRSGQGKVYP LINRRRFQQM DVLEGLNVLV TISGKKDKLR VYYLSWLRNK
1010 1020 1030 1040 1050
ILHNDPEVEK KQGWTTVGDL EGCVHYKVVK YERIKFLVIA LKSSVEVYAW
1060 1070 1080 1090 1100
APKPYHKFMA FKSFGELVHK PLLVDLTVEE GQRLKVIYGS CAGFHAVDVD
1110 1120 1130 1140 1150
SGSVYDIYLP THIQCSIKPH AIIILPNTDG MELLVCYEDE GVYVNTYGRI
1160 1170 1180 1190 1200
TKDVVLQWGE MPTSVAYIRS NQTMGWGEKA IEIRSVETGH LDGVFMHKRA
1210 1220 1230 1239
QRLKFLCERN DKVFFASVRS GGSSQVYFMT LGRTSLLSW

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-6106 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP4K4
Peptide Sequence
VYPLINR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
958
Peptide End
964
CPTAC ID
non-CPTAC-6106
Peptide Molecular Mass
873.5072
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6.5 4.8 4.6 7.2 4.6 6.1 9.7 6.6 7.6 16 16 17
y3 (1+) 6.8 5.5 6.2 7.4 7.4 5.4 10 9.2 8.2 16 16 17
y4 (1+) 10.7 5.1 6 8 5.8 5.5 13.4 7.7 8.1 16 16 17
y5 (1+) 5.7 5.3 7.2 7.7 5.4 6 9.6 7.6 9.4 16 16 17
y5 (2+) 8.1 6.3 2.7 8.1 3.8 5.1 11.5 7.4 5.8 16 16 17
sum 2.9 3.1 3.4 3 3 4 4.2 4.3 5.2 16 16 17

Stability

Data source: Panorama

view larger image
Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
b2 (1+) 5.2 6.4 8.7 3.6 5.2
y3 (1+) 7 3.9 10.4 7 10.4
y4 (1+) 9 2.4 6.5 5.7 6.9
y5 (1+) 4.4 3.6 8.6 5.9 8.6
y5 (2+) 5.5 4.2 4.4 4.8 3
sum 1.8 1.3 2.7 2.4 0.6
Fragment ion / Transition all_intra_CV all_inter_CV
b2 (1+) 5.9 5.8
y3 (1+) 7.1 9.8
y4 (1+) 5.5 7.5
y5 (1+) 5.8 7.3
y5 (2+) 4.3 5.1
sum 1.7 3

Endogenous

Data source: Panorama

view larger image
Fragment ion / Transition intra_CV inter_CV total_CV
b2 (1+) 92 74.1 118.1
y3 (1+) 36.1 75.9 84
y4 (1+) 84.1 105.1 134.6
y5 (1+) 29.2 83.5 88.5
y5 (2+) 24.2 49.6 55.2
sum 0.9 40.3 40.3

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-1049 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP4K4
Peptide Sequence
DSPLQGSGQQNSQAGQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
638
Peptide End
654
CPTAC ID
CPTAC-1049
Peptide Molecular Mass
1,756.8089
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 21.9 8 8.8 21.1 8.9 9.3 30.4 12 12.8 15 15 15
y6 (1+) 12.3 12 8.1 14.5 7.5 11.8 19 14.2 14.3 15 15 15
y4 (1+) 19.7 6.5 13.2 18.6 6.2 11.9 27.1 9 17.8 15 15 15
sum 11.6 6.3 7.1 11.4 5 7.2 16.3 8 10.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-866 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP4K4
Peptide Sequence
VYPLINR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
958
Peptide End
964
CPTAC ID
CPTAC-866
Peptide Molecular Mass
873.5072
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 5.8 4 4.9 8.6 5.7 6.4 10.4 7 8.1 15 15 15
y4 (1+) 8 5.7 3.8 8.5 8.5 6.5 11.7 10.2 7.5 15 15 15
y5 (1+) 5.3 2.3 3.7 8.5 4.3 5.3 10 4.9 6.5 15 15 15
y6 (1+) 21.6 12.7 10.3 23.8 12 12.1 32.1 17.5 15.9 15 15 15
b6 (1+) 62.9 39.4 34.7 87 58.2 47.7 107.4 70.3 59 15 15 15
b5 (1+) 51.6 49 26.3 99.4 47.9 24.5 112 68.5 35.9 15 15 15
b4 (1+) 64.4 51 37.3 60.3 73.8 35.6 88.2 89.7 51.6 15 15 15
b3 (1+) 23.3 32.7 13.8 33.8 31.7 12.3 41.1 45.5 18.5 15 15 15
sum 4.4 2.9 3.5 7.1 5.3 5.3 8.4 6 6.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments