Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

RPL27A

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
RPL27A N/A
Sequence Length (AA) Molecular Weight (Da)
148 16561
Protein Name
60S ribosomal protein L27a
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGLHH HRINFDKYHP
60 70 80 90 100
GYFGKVGMKH YHLKRNQSFC PTVNLDKLWT LVSEQTRVNA AKNKTGAAPI
110 120 130 140 148
IDVVRSGYYK VLGKGKLPKQ PVIVKAKFFS RRAEEKIKSV GGACVLVA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-6120 Collapse assay details

Data source: Panorama

Official Gene Symbol
RPL27A
Peptide Sequence
TGAAPIIDVVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
95
Peptide End
105
CPTAC ID
non-CPTAC-6120
Peptide Molecular Mass
1,110.6397
Species
Homo sapiens (Human)
Assay Type
Direct MRM-MS
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 7.8 6.4 7.9 10.7 6.7 8.7 13.2 9.3 11.8 16 16 17
y7 (1+) 7.3 6.8 6.1 6.6 7.5 5.1 9.8 10.1 8 16 16 17
y7 (2+) 14.3 7 7.9 16.1 7.7 6.4 21.5 10.4 10.2 16 16 17
sum 4.7 3.1 4.9 5.5 3.7 4.6 7.2 4.8 6.7 16 16 17

Stability

Data source: Panorama

view larger image
Fragment ion / Transition control_intra_CV actual_temp_intra_CV frozen_intra_CV FTx1_intra_CV FTx2_intra_CV
y7 (1+) 9 3 4.7 3.4 11.9
b3 (1+) 7 7.4 3.5 6 2.8
y7 (2+) 7 5.5 5.7 4.4 16.2
sum 4.9 2.5 3.3 3.7 3.6
Fragment ion / Transition all_intra_CV all_inter_CV
y7 (1+) 5.9 7
b3 (1+) 5.7 4.2
y7 (2+) 7.4 6.9
sum 3.4 3.5

Endogenous

Data source: Panorama

view larger image
Fragment ion / Transition intra_CV inter_CV total_CV
y7 (1+) 8.6 21.5 23.2
b3 (1+) 7.5 18.3 19.8
y7 (2+) 15.3 20.9 25.9
sum 2.6 19.2 19.4

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments