PPP6R3, CPTAC-1002 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PPP6R3	C11orf23, KIAA1558, PP6R3, SAPL, SAPS3

Sequence Length (AA)	Molecular Weight (Da)
873	97669

Protein Name
Serine/threonine-protein phosphatase 6 regulatory subunit 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MFWKFDLHSS	SHIDTLLERE	DVTLKELMDE	EDVLQECKAQ	NRKLIEFLLK
60	70	80	90	100
AECLEDLVSF	IIEEPPQDMD	EKIRYKYPNI	SCELLTSDVS	QMNDRLGEDE
110	120	130	140	150
SLLMKLYSFL	LNDSPLNPLL	ASFFSKVLSI	LISRKPEQIV	DFLKKKHDFV
160	170	180	190	200
DLIIKHIGTS	AIMDLLLRLL	TCIEPPQPRQ	DVLNWLNEEK	IIQRLVEIVH
210	220	230	240	250
PSQEEDRHSN	ASQSLCEIVR	LSRDQMLQIQ	NSTEPDPLLA	TLEKQEIIEQ
260	270	280	290	300
LLSNIFHKEK	NESAIVSAIQ	ILLTLLETRR	PTFEGHIEIC	PPGMSHSACS
310	320	330	340	350
VNKSVLEAIR	GRLGSFHELL	LEPPKKSVMK	TTWGVLDPPV	GNTRLNVIRL
360	370	380	390	400
ISSLLQTNTS	SINGDLMELN	SIGVILNMFF	KYTWNNFLHT	QVEICIALIL
410	420	430	440	450
ASPFENTENA	TITDQDSTGD	NLLLKHLFQK	CQLIERILEA	WEMNEKKQAE
460	470	480	490	500
GGRRHGYMGH	LTRIANCIVH	STDKGPNSAL	VQQLIKDLPD	EVRERWETFC
510	520	530	540	550
TSSLGETNKR	NTVDLVTTCH	IHSSSDDEID	FKETGFSQDS	SLQQAFSDYQ
560	570	580	590	600
MQQMTSNFID	QFGFNDEKFA	DQDDIGNVSF	DRVSDINFTL	NTNESGNIAL
610	620	630	640	650
FEACCKERIQ	QFDDGGSDEE	DIWEEKHIAF	TPESQRRSSS	GSTDSEESTD
660	670	680	690	700
SEEEDGAKQD	LFEPSSANTE	DKMEVDLSEP	PNWSANFDVP	METTHGAPLD
710	720	730	740	750
SVGSDVWSTE	EPMPTKETGW	ASFSEFTSSL	STKDSLRSNS	PVEMETSTEP
760	770	780	790	800
MDPLTPSAAA	LAVQPEAAGS	VAMEASSDGE	EDAESTDKVT	ETVMNGGMKE
810	820	830	840	850
TLSLTVDAKT	ETAVFKSEEG	KLSTSQDAAC	KDAEECPETA	EAKCAAPRPP
860	870	873
SSSPEQRTGQ	PSAPGDTSVN	GPV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1002 Collapse assay details

Data source: Panorama

Official Gene Symbol: PPP6R3
Peptide Modified Sequence: IQQFDDGGS[+80.0]DEEDIWEEK
Modification Type: Phospho (ST)
Protein - Site of Modification: 617
Peptide - Site of Modification: 9
Peptide Start: 609
Peptide End: 626

CPTAC ID: CPTAC-1002
Peptide Molecular Mass: 2,218.8580
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y16-98 (2+)	43.8	18.2	6.7	44.3	29.6	7	62.3	34.7	9.7	15	24	15
y17 (2+)	49.5	33.4	15.7	51.2	37.9	15.3	71.2	50.5	21.9	15	24	15
y4 (1+)	20.2	7.7	5.4	30.8	20	9	36.8	21.4	10.5	15	24	15
y8 (1+)	55.9	25.3	10.9	61.3	35.1	14.7	83	43.3	18.3	15	24	15
sum	30.9	6.5	5.1	29.7	21.5	8.2	42.9	22.5	9.7	15	24	15

PPP6R3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1002 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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PPP6R3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1002 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments