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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PPP6R3 C11orf23, KIAA1558, PP6R3, SAPL, SAPS3
Sequence Length (AA) Molecular Weight (Da)
873 97669
Protein Name
Serine/threonine-protein phosphatase 6 regulatory subunit 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MFWKFDLHSS SHIDTLLERE DVTLKELMDE EDVLQECKAQ NRKLIEFLLK
60 70 80 90 100
AECLEDLVSF IIEEPPQDMD EKIRYKYPNI SCELLTSDVS QMNDRLGEDE
110 120 130 140 150
SLLMKLYSFL LNDSPLNPLL ASFFSKVLSI LISRKPEQIV DFLKKKHDFV
160 170 180 190 200
DLIIKHIGTS AIMDLLLRLL TCIEPPQPRQ DVLNWLNEEK IIQRLVEIVH
210 220 230 240 250
PSQEEDRHSN ASQSLCEIVR LSRDQMLQIQ NSTEPDPLLA TLEKQEIIEQ
260 270 280 290 300
LLSNIFHKEK NESAIVSAIQ ILLTLLETRR PTFEGHIEIC PPGMSHSACS
310 320 330 340 350
VNKSVLEAIR GRLGSFHELL LEPPKKSVMK TTWGVLDPPV GNTRLNVIRL
360 370 380 390 400
ISSLLQTNTS SINGDLMELN SIGVILNMFF KYTWNNFLHT QVEICIALIL
410 420 430 440 450
ASPFENTENA TITDQDSTGD NLLLKHLFQK CQLIERILEA WEMNEKKQAE
460 470 480 490 500
GGRRHGYMGH LTRIANCIVH STDKGPNSAL VQQLIKDLPD EVRERWETFC
510 520 530 540 550
TSSLGETNKR NTVDLVTTCH IHSSSDDEID FKETGFSQDS SLQQAFSDYQ
560 570 580 590 600
MQQMTSNFID QFGFNDEKFA DQDDIGNVSF DRVSDINFTL NTNESGNIAL
610 620 630 640 650
FEACCKERIQ QFDDGGSDEE DIWEEKHIAF TPESQRRSSS GSTDSEESTD
660 670 680 690 700
SEEEDGAKQD LFEPSSANTE DKMEVDLSEP PNWSANFDVP METTHGAPLD
710 720 730 740 750
SVGSDVWSTE EPMPTKETGW ASFSEFTSSL STKDSLRSNS PVEMETSTEP
760 770 780 790 800
MDPLTPSAAA LAVQPEAAGS VAMEASSDGE EDAESTDKVT ETVMNGGMKE
810 820 830 840 850
TLSLTVDAKT ETAVFKSEEG KLSTSQDAAC KDAEECPETA EAKCAAPRPP
860 870 873
SSSPEQRTGQ PSAPGDTSVN GPV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1002 Collapse assay details

Data source: Panorama

Official Gene Symbol
PPP6R3
Peptide Modified Sequence
IQQFDDGGS[+80.0]DEEDIWEEK
Modification Type
Phospho (ST)
Protein - Site of Modification
617
Peptide - Site of Modification
9
Peptide Start
609
Peptide End
626
CPTAC ID
CPTAC-1002
Peptide Molecular Mass
2,218.8580
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y16-98 (2+) 43.8 18.2 6.7 44.3 29.6 7 62.3 34.7 9.7 15 24 15
y17 (2+) 49.5 33.4 15.7 51.2 37.9 15.3 71.2 50.5 21.9 15 24 15
y4 (1+) 20.2 7.7 5.4 30.8 20 9 36.8 21.4 10.5 15 24 15
y8 (1+) 55.9 25.3 10.9 61.3 35.1 14.7 83 43.3 18.3 15 24 15
sum 30.9 6.5 5.1 29.7 21.5 8.2 42.9 22.5 9.7 15 24 15


Additional Resources and Comments