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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

SH2D4A

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SH2D4A PPP1R38, SH2A
Sequence Length (AA) Molecular Weight (Da)
454 52727
Protein Name
SH2 domain-containing protein 4A
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLKQILSEMY IDPDLLAELS EEQKQILFFK MREEQIRRWK EREAAMERKE
60 70 80 90 100
SLPVKPRPKK ENGKSVHWKL GADKEVWVWV MGEHHLDKPY DVLCNEIIAE
110 120 130 140 150
RARLKAEQEA EEPRKTHSEE FTNSLKTKSQ YHDLQAPDNQ QTKDIWKKVA
160 170 180 190 200
EKEELEQGSR PAPTLEEEKI RSLSSSSRNI QQMLADSINR MKAYAFHQKK
210 220 230 240 250
ESMKKKQDEE INQIEEERTK QICKSWKEDS EWQASLRKSK AADEKRRSLA
260 270 280 290 300
KQAREDYKRL SLGAQKGRGG ERLQSPLRVP QKPERPPLPP KPQFLNSGAY
310 320 330 340 350
PQKPLRNQGV VRTLSSSAQE DIIRWFKEEQ LPLRAGYQKT SDTIAPWFHG
360 370 380 390 400
ILTLKKANEL LLSTGMPGSF LIRVSERIKG YALSYLSEDG CKHFLIDASA
410 420 430 440 450
DAYSFLGVDQ LQHATLADLV EYHKEEPITS LGKELLLYPC GQQDQLPDYL
454
ELFE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1003 Collapse assay details

Data source: Panorama

Official Gene Symbol
SH2D4A
Peptide Modified Sequence
TLS[+80.0]SSAQEDIIR
Modification Type
Phospho (ST)
Protein - Site of Modification
315
Peptide - Site of Modification
3
Peptide Start
313
Peptide End
324
CPTAC ID
CPTAC-1003
Peptide Molecular Mass
1,398.6392
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10-98 (2+) 36.5 9.7 8.6 32.9 16.1 17.8 49.1 18.8 19.8 15 24 15
y6 (1+) 38.9 10.9 11.9 24.1 14.4 15.2 45.8 18.1 19.3 15 24 15
y10 (1+) 34.9 7 6.1 18.1 10.5 11.2 39.3 12.6 12.8 15 24 15
sum 31.7 6.8 7.9 18.7 12.2 14.4 36.8 14 16.4 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments