SH3BP1, CPTAC-1004 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SH3BP1	N/A

Sequence Length (AA)	Molecular Weight (Da)
701	75713

Protein Name
SH3 domain-binding protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MMKRQLHRMR	QLAQTGSLGR	TPETAEFLGE	DLLQVEQRLE	PAKRAAHNIH
60	70	80	90	100
KRLQACLQGQ	SGADMDKRVK	KLPLMALSTT	MAESFKELDP	DSSMGKALEM
110	120	130	140	150
SCAIQNQLAR	ILAEFEMTLE	RDVLQPLSRL	SEEELPAILK	HKKSLQKLVS
160	170	180	190	200
DWNTLKSRLS	QATKNSGSSQ	GLGGSPGSHS	HTTMANKVET	LKEEEEELKR
210	220	230	240	250
KVEQCRDEYL	ADLYHFVTKE	DSYANYFIRL	LEIQADYHRR	SLSSLDTALA
260	270	280	290	300
ELRENHGQAD	HSPSMTATHF	PRVYGVSLAT	HLQELGREIA	LPIEACVMML
310	320	330	340	350
LSEGMKEEGL	FRLAAGASVL	KRLKQTMASD	PHSLEEFCSD	PHAVAGALKS
360	370	380	390	400
YLRELPEPLM	TFDLYDDWMR	AASLKEPGAR	LQALQEVCSR	LPPENLSNLR
410	420	430	440	450
YLMKFLARLA	EEQEVNKMTP	SNIAIVLGPN	LLWPPEKEGD	QAQLDAASVS
460	470	480	490	500
SIQVVGVVEA	LIQSADTLFP	GDINFNVSGL	FSAVTLQDTV	SDRLASEELP
510	520	530	540	550
STAVPTPATT	PAPAPAPAPA	PAPALASAAT	KERTESEVPP	RPASPKVTRS
560	570	580	590	600
PPETAAPVED	MARRTKRPAP	ARPTMPPPQV	SGSRSSPPAP	PLPPGSGSPG
610	620	630	640	650
TPQALPRRLV	GSSLRAPTVP	PPLPPTPPQP	ARRQSRRSPA	SPSPASPGPA
660	670	680	690	700
SPSPVSLSNP	AQVDLGAATA	EGGAPEAISG	VPTPPAIPPQ	PRPRSLASET
701
N

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1004 Collapse assay details

Data source: Panorama

Official Gene Symbol: SH3BP1
Peptide Modified Sequence: TESEVPPRPAS[+80.0]PK
Modification Type: Phospho (ST)
Protein - Site of Modification: 544
Peptide - Site of Modification: 11
Peptide Start: 534
Peptide End: 546

CPTAC ID: CPTAC-1004
Peptide Molecular Mass: 1,473.6865
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (2+)	29.4	11.5	10.4	47.1	10.1	13.3	55.5	15.3	16.9	15	24	15
y8-98 (1+)	17.4	9	9.7	20.3	8.6	12.1	26.7	12.4	15.5	15	24	15
y8 (1+)	20.4	9.7	9.6	19.9	10.4	11.8	28.5	14.2	15.2	15	24	15
sum	15.7	9.4	9.6	20.4	9.4	12.1	25.7	13.3	15.4	15	24	15

SH3BP1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1004 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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SH3BP1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1004 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments