SLC9A1, CPTAC-1005 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SLC9A1	APNH1, NHE1

Sequence Length (AA)	Molecular Weight (Da)
815	90763

Protein Name
Sodium/hydrogen exchanger 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVLRSGICGL	SPHRIFPSLL	VVVALVGLLP	VLRSHGLQLS	PTASTIRSSE
60	70	80	90	100
PPRERSIGDV	TTAPPEVTPE	SRPVNHSVTD	HGMKPRKAFP	VLGIDYTHVR
110	120	130	140	150
TPFEISLWIL	LACLMKIGFH	VIPTISSIVP	ESCLLIVVGL	LVGGLIKGVG
160	170	180	190	200
ETPPFLQSDV	FFLFLLPPII	LDAGYFLPLR	QFTENLGTIL	IFAVVGTLWN
210	220	230	240	250
AFFLGGLMYA	VCLVGGEQIN	NIGLLDNLLF	GSIISAVDPV	AVLAVFEEIH
260	270	280	290	300
INELLHILVF	GESLLNDAVT	VVLYHLFEEF	ANYEHVGIVD	IFLGFLSFFV
310	320	330	340	350
VALGGVLVGV	VYGVIAAFTS	RFTSHIRVIE	PLFVFLYSYM	AYLSAELFHL
360	370	380	390	400
SGIMALIASG	VVMRPYVEAN	ISHKSHTTIK	YFLKMWSSVS	ETLIFIFLGV
410	420	430	440	450
STVAGSHHWN	WTFVISTLLF	CLIARVLGVL	GLTWFINKFR	IVKLTPKDQF
460	470	480	490	500
IIAYGGLRGA	IAFSLGYLLD	KKHFPMCDLF	LTAIITVIFF	TVFVQGMTIR
510	520	530	540	550
PLVDLLAVKK	KQETKRSINE	EIHTQFLDHL	LTGIEDICGH	YGHHHWKDKL
560	570	580	590	600
NRFNKKYVKK	CLIAGERSKE	PQLIAFYHKM	EMKQAIELVE	SGGMGKIPSA
610	620	630	640	650
VSTVSMQNIH	PKSLPSERIL	PALSKDKEEE	IRKILRNNLQ	KTRQRLRSYN
660	670	680	690	700
RHTLVADPYE	EAWNQMLLRR	QKARQLEQKI	NNYLTVPAHK	LDSPTMSRAR
710	720	730	740	750
IGSDPLAYEP	KEDLPVITID	PASPQSPESV	DLVNEELKGK	VLGLSRDPAK
760	770	780	790	800
VAEEDEDDDG	GIMMRSKETS	SPGTDDVFTP	APSDSPSSQR	IQRCLSDPGP
810	815
HPEPGEGEPF	FPKGQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1005 Collapse assay details

Data source: Panorama

Official Gene Symbol: SLC9A1
Peptide Modified Sequence: C[+57.0]LS[+80.0]DPGPHPEPGEGEPFFPK
Modification Type: Carbamidomethyl Cysteine, Phospho (ST)
Protein - Site of Modification: 794, 796
Peptide - Site of Modification: 1, 3
Peptide Start: 794
Peptide End: 813

CPTAC ID: CPTAC-1005
Peptide Molecular Mass: 2,272.9500
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y16 (2+)	22.1	14.7	12.4	23.1	18.8	10.9	32	23.9	16.5	15	24	15
y18-98 (2+)	50.4	24.4	24.3	36.6	22.8	27.6	62.3	33.4	36.8	15	24	15
y18 (2+)	27.6	24	17.3	24.5	24.5	11.1	36.9	34.3	20.6	15	24	15
sum	25.2	13.7	13.7	21.3	17.8	10.7	33	22.5	17.4	15	24	15

SLC9A1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1005 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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SLC9A1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1005 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments