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Overview Data source: UniProt

Official Gene Symbol Other Aliases
SLC9A1 APNH1,NHE1
Sequence Length (AA) Molecular Weight (Da)
815 90763
Protein Name
Sodium/hydrogen exchanger 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVLRSGICGL SPHRIFPSLL VVVALVGLLP VLRSHGLQLS PTASTIRSSE
60 70 80 90 100
PPRERSIGDV TTAPPEVTPE SRPVNHSVTD HGMKPRKAFP VLGIDYTHVR
110 120 130 140 150
TPFEISLWIL LACLMKIGFH VIPTISSIVP ESCLLIVVGL LVGGLIKGVG
160 170 180 190 200
ETPPFLQSDV FFLFLLPPII LDAGYFLPLR QFTENLGTIL IFAVVGTLWN
210 220 230 240 250
AFFLGGLMYA VCLVGGEQIN NIGLLDNLLF GSIISAVDPV AVLAVFEEIH
260 270 280 290 300
INELLHILVF GESLLNDAVT VVLYHLFEEF ANYEHVGIVD IFLGFLSFFV
310 320 330 340 350
VALGGVLVGV VYGVIAAFTS RFTSHIRVIE PLFVFLYSYM AYLSAELFHL
360 370 380 390 400
SGIMALIASG VVMRPYVEAN ISHKSHTTIK YFLKMWSSVS ETLIFIFLGV
410 420 430 440 450
STVAGSHHWN WTFVISTLLF CLIARVLGVL GLTWFINKFR IVKLTPKDQF
460 470 480 490 500
IIAYGGLRGA IAFSLGYLLD KKHFPMCDLF LTAIITVIFF TVFVQGMTIR
510 520 530 540 550
PLVDLLAVKK KQETKRSINE EIHTQFLDHL LTGIEDICGH YGHHHWKDKL
560 570 580 590 600
NRFNKKYVKK CLIAGERSKE PQLIAFYHKM EMKQAIELVE SGGMGKIPSA
610 620 630 640 650
VSTVSMQNIH PKSLPSERIL PALSKDKEEE IRKILRNNLQ KTRQRLRSYN
660 670 680 690 700
RHTLVADPYE EAWNQMLLRR QKARQLEQKI NNYLTVPAHK LDSPTMSRAR
710 720 730 740 750
IGSDPLAYEP KEDLPVITID PASPQSPESV DLVNEELKGK VLGLSRDPAK
760 770 780 790 800
VAEEDEDDDG GIMMRSKETS SPGTDDVFTP APSDSPSSQR IQRCLSDPGP
810 815
HPEPGEGEPF FPKGQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1005 Collapse assay details

Data source: Panorama

Official Gene Symbol
SLC9A1
Peptide Modified Sequence
C[+57.0]LS[+80.0]DPGPHPEPGEGEPFFPK
Modification Type
Carbamidomethyl Cysteine, Phospho (ST)
Protein - Site of Modification
794, 796
Peptide - Site of Modification
1, 3
Peptide Start
794
Peptide End
813
CPTAC ID
CPTAC-1005
Peptide Molecular Mass
2,272.9500
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y16 (2+) 22.1 14.7 12.4 23.1 18.8 10.9 32 23.9 16.5 15 24 15
y18-98 (2+) 50.4 24.4 24.3 36.6 22.8 27.6 62.3 33.4 36.8 15 24 15
y18 (2+) 27.6 24 17.3 24.5 24.5 11.1 36.9 34.3 20.6 15 24 15
sum 25.2 13.7 13.7 21.3 17.8 10.7 33 22.5 17.4 15 24 15

Additional Resources and Comments