ZC3H11A, CPTAC-1020 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ZC3H11A	KIAA0663, ZC3HDC11A

Sequence Length (AA)	Molecular Weight (Da)
810	89131

Protein Name
Zinc finger CCCH domain-containing protein 11A

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPNQGEDCYF	FFYSTCTKGD	SCPFRHCEAA	IGNETVCTLW	QEGRCFRQVC
60	70	80	90	100
RFRHMEIDKK	RSEIPCYWEN	QPTGCQKLNC	AFHHNRGRYV	DGLFLPPSKT
110	120	130	140	150
VLPTVPESPE	EEVKASQLSV	QQNKLSVQSN	PSPQLRSVMK	VESSENVPSP
160	170	180	190	200
THPPVVINAA	DDDEDDDDQF	SEEGDETKTP	TLQPTPEVHN	GLRVTSVRKP
210	220	230	240	250
AVNIKQGECL	NFGIKTLEEI	KSKKMKEKSK	KQGEGSSGVS	SLLLHPEPVP
260	270	280	290	300
GPEKENVRTV	VRTVTLSTKQ	GEEPLVRLSL	TERLGKRKFS	AGGDSDPPLK
310	320	330	340	350
RSLAQRLGKK	VEAPETNIDK	TPKKAQVSKS	LKERLGMSAD	PDNEDATDKV
360	370	380	390	400
NKVGEIHVKT	LEEILLERAS	QKRGELQTKL	KTEGPSKTDD	STSGARSSST
410	420	430	440	450
IRIKTFSEVL	AEKKHRQQEA	ERQKSKKDTT	CIKLKIDSEI	KKTVVLPPIV
460	470	480	490	500
ASRGQSEEPA	GKTKSMQEVH	IKTLEEIKLE	KALRVQQSSE	SSTSSPSQHE
510	520	530	540	550
ATPGARRLLR	ITKRTGMKEE	KNLQEGNEVD	SQSSIRTEAK	EASGETTGVD
560	570	580	590	600
ITKIQVKRCE	TMREKHMQKQ	QEREKSVLTP	LRGDVASCNT	QVAEKPVLTA
610	620	630	640	650
VPGITRHLTK	RLPTKSSQKV	EVETSGIGDS	LLNVKCAAQT	LEKRGKAKPK
660	670	680	690	700
VNVKPSVVKV	VSSPKLAPKR	KAVEMHAAVI	AAVKPLSSSS	VLQEPPAKKA
710	720	730	740	750
AVAVVPLVSE	DKSVTVPEAE	NPRDSLVLPP	TQSSSDSSPP	EVSGPSSSQM
760	770	780	790	800
SMKTRRLSSA	STGKPPLSVE	DDFEKLIWEI	SGGKLEAEID	LDPGKDEDDL
810
LLELSEMIDS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1020 Collapse assay details

Data source: Panorama

Official Gene Symbol: ZC3H11A
Peptide Modified Sequence: TVLPTVPES[+80.0]PEEEVK
Modification Type: Phospho (ST)
Protein - Site of Modification: 108
Peptide - Site of Modification: 9
Peptide Start: 100
Peptide End: 114

CPTAC ID: CPTAC-1020
Peptide Molecular Mass: 1,732.8172
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (2+)	40.7	10.9	10.8	22.8	15.8	10.6	46.7	19.2	15.1	15	24	15
y13 (2+)	40	12.9	14.3	31	16.9	18	50.6	21.3	23	15	24	15
y9 (1+)	42.2	13	8.9	27.5	17.5	11.1	50.4	21.8	14.2	15	24	15
sum	37.9	11	7.1	25.1	15.9	8.1	45.5	19.3	10.8	15	24	15

ZC3H11A

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1020 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

ZC3H11A

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1020 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments