ZCCHC8, CPTAC-1021 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ZCCHC8	N/A

Sequence Length (AA)	Molecular Weight (Da)
707	78577

Protein Name
Zinc finger CCHC domain-containing protein 8

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAEVYFGDL	ELFEPFDHPE	ESIPKPVHTR	FKDDDGDEED	ENGVGDAELR
60	70	80	90	100
ERLRQCEETI	EQLRAENQEL	KRKLNILTRP	SGILVNDTKL	DGPILQILFM
110	120	130	140	150
NNAISKQYHQ	EIEEFVSNLV	KRFEEQQKND	VEKTSFNLLP	QPSSIVLEED
160	170	180	190	200
HKVEESCAIK	NNKEAFSVVG	SVLYFTNFCL	DKLGQPLLNE	NPQLSEGWEI
210	220	230	240	250
PKYHQVFSHI	VSLEGQEIQV	KAKRPKPHCF	NCGSEEHQMK	DCPMPRNAAR
260	270	280	290	300
ISEKRKEYMD	ACGEANNQNF	QQRYHAEEVE	ERFGRFKPGV	ISEELQDALG
310	320	330	340	350
VTDKSLPPFI	YRMRQLGYPP	GWLKEAELEN	SGLALYDGKD	GTDGETEVGE
360	370	380	390	400
IQQNKSVTYD	LSKLVNYPGF	NISTPRGIPD	EWRIFGSIPM	QACQQKDVFA
410	420	430	440	450
NYLTSNFQAP	GVKSGNKRSS	SHSSPGSPKK	QKNESNSAGS	PADMELDSDM
460	470	480	490	500
EVPHGSQSSE	SFQFQPPLPP	DTPPLPRGTP	PPVFTPPLPK	GTPPLTPSDS
510	520	530	540	550
PQTRTASGAV	DEDALTLEEL	EEQQRRIWAA	LEQAESVNSD	SDVPVDTPLT
560	570	580	590	600
GNSVASSPCP	NELDLPVPEG	KTSEKQTLDE	PEVPEIFTKK	SEAGHASSPD
610	620	630	640	650
SEVTSLCQKE	KAELAPVNTE	GALLDNGSVV	PNCDISNGGS	QKLFPADTSP
660	670	680	690	700
STATKIHSPI	PDMSKFATGI	TPFEFENMAE	STGMYLRIRS	LLKNSPRNQQ
707
KNKKASE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1021 Collapse assay details

Data source: Panorama

Official Gene Symbol: ZCCHC8
Peptide Modified Sequence: GT[+80.0]PPPVFTPPLPK
Modification Type: Phospho (ST)
Protein - Site of Modification: 479
Peptide - Site of Modification: 2
Peptide Start: 478
Peptide End: 490

CPTAC ID: CPTAC-1021
Peptide Molecular Mass: 1,426.7262
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (2+)	33.2	7.7	5.6	18.9	9.7	6.1	38.2	12.4	8.3	15	24	15
y5 (1+)	39	14.8	15.2	26.5	17.4	17.2	47.2	22.8	23	15	24	15
y10 (1+)	35.6	9.5	8.8	17.8	8.3	9.1	39.8	12.6	12.7	15	24	15
sum	33.3	7.8	5.8	18.8	9.6	6.2	38.2	12.4	8.5	15	24	15

ZCCHC8

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1021 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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ZCCHC8

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1021 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments