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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

CRACR2A

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CRACR2A EFCAB4B
Sequence Length (AA) Molecular Weight (Da)
395 45592
Protein Name
EF-hand calcium-binding domain-containing protein 4B
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAPDGRVVS RPQRLGQGSG QGPKGSGACL HPLDSLEQKE TQEQTSGQLV
60 70 80 90 100
MLRKAQEFFQ TCDAEGKGFI ARKDMQRLHK ELPLSLEELE DVFDALDADG
110 120 130 140 150
NGYLTPQEFT TGFSHFFFSQ NNPSQEDAGE QVAQRHEEKV YLSRGDEDLG
160 170 180 190 200
DMGEDEEAQF RMLMDRLGAQ KVLEDESDVK QLWLQLKKEE PHLLSNFEDF
210 220 230 240 250
LTRIISQLQE AHEEKNELEC ALKRKIAAYD EEIQHLYEEM EQQIKSEKEQ
260 270 280 290 300
FLLKDTERFQ ARSQELEQKL LCKEQELEQL TQKQKRLEGQ CTALHHDKHE
310 320 330 340 350
TKAENTKLKL TNQELARELE RTSWELQDAQ QQLESLQQEA CKLHQEKEME
360 370 380 390 395
VYRVTESLQR EKAGLLKQLD FLRCVGGHWP VLRAPPRSLG SEGPV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1026 Collapse assay details

Data source: Panorama

Official Gene Symbol
CRACR2A
Peptide Modified Sequence
IIS[+80.0]VEEDPLPQLLDGGFEQPLSK
Modification Type
Phospho (ST)
Protein - Site of Modification
3
Peptide - Site of Modification
3
Peptide Start
1
Peptide End
23
CPTAC ID
CPTAC-1026
Peptide Molecular Mass
2,603.2772
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y14 (2+) 39 17.7 15.7 35.3 47.6 32.9 52.6 50.8 36.5 12 20 13
y4 (1+) 32.6 17.9 17.2 32.8 46.1 32.9 46.2 49.5 37.1 12 20 13
y10 (1+) 61.8 19.1 15 55.6 45.7 32 83.1 49.5 35.3 12 20 13
sum 37.5 17.1 15.9 34.3 46.3 32.3 50.8 49.4 36 12 20 13

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments