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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

GPN1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
GPN1 MBDIN, RPAP4, XAB1
Sequence Length (AA) Molecular Weight (Da)
374 41740
Protein Name
GPN-loop GTPase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAASAAAAEL QASGGPRHPV CLLVLGMAGS GKTTFVQRLT GHLHAQGTPP
60 70 80 90 100
YVINLDPAVH EVPFPANIDI RDTVKYKEVM KQYGLGPNGG IVTSLNLFAT
110 120 130 140 150
RFDQVMKFIE KAQNMSKYVL IDTPGQIEVF TWSASGTIIT EALASSFPTV
160 170 180 190 200
VIYVMDTSRS TNPVTFMSNM LYACSILYKT KLPFIVVMNK TDIIDHSFAV
210 220 230 240 250
EWMQDFEAFQ DALNQETTYV SNLTRSMSLV LDEFYSSLRV VGVSAVLGTG
260 270 280 290 300
LDELFVQVTS AAEEYEREYR PEYERLKKSL ANAESQQQRE QLERLRKDMG
310 320 330 340 350
SVALDAGTAK DSLSPVLHPS DLILTRGTLD EEDEEADSDT DDIDHRVTEE
360 370 374
SHEEPAFQNF MQESMAQYWK RNNK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1027 Collapse assay details

Data source: Panorama

Official Gene Symbol
GPN1
Peptide Modified Sequence
DSLS[+80.0]PVLHPSDLILTR
Modification Type
Phospho (ST)
Protein - Site of Modification
314
Peptide - Site of Modification
4
Peptide Start
311
Peptide End
326
CPTAC ID
CPTAC-1027
Peptide Molecular Mass
1,841.9288
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 31 12.5 14.2 37.1 27.4 15.6 48.3 30.1 21.1 15 24 15
y13 (2+) 35.7 12.6 12.9 32.1 33.7 19.9 48 36 23.7 15 24 15
y14 (2+) 30.2 17.9 12.4 28.9 33.9 12.1 41.8 38.3 17.3 15 24 15
sum 31 11.2 11 32.1 29.8 14.8 44.6 31.8 18.4 15 24 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments