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Overview Data source: UniProt

Official Gene Symbol Other Aliases
VAMP4 N/A
Sequence Length (AA) Molecular Weight (Da)
141 16397
Protein Name
Vesicle-associated membrane protein 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN
60 70 80 90 100
DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN
110 120 130 140 141
RSKQLRRQMW WRGCKIKAIM ALVAAILLLV IIILIVMKYR T

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1035 Collapse assay details

Data source: Panorama

Official Gene Symbol
VAMP4
Peptide Modified Sequence
NLLEDDS[+80.0]DEEEDFFLR
Modification Type
Phospho (ST)
Protein - Site of Modification
30
Peptide - Site of Modification
7
Peptide Start
24
Peptide End
39
CPTAC ID
CPTAC-1035
Peptide Molecular Mass
2,064.8201
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y14 (2+) 47.6 13.2 21.7 43.4 42.8 32.1 64.4 44.8 38.7 12 20 13
y6 (1+) 23.9 17.3 10.6 34.6 41.1 20.5 42.1 44.6 23.1 12 20 13
y8 (1+) 27.8 14.5 12.3 36.6 48.4 20 46 50.5 23.5 12 20 13
sum 26.6 11 11.3 28.9 42.3 21.7 39.3 43.7 24.5 12 20 13

Additional Resources and Comments