STK17B, CPTAC-1034 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STK17B	DRAK2

Sequence Length (AA)	Molecular Weight (Da)
372	42344

Protein Name
Serine/threonine-protein kinase 17B

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSRRRFDCRS	ISGLLTTTPQ	IPIKMENFNN	FYILTSKELG	RGKFAVVRQC
60	70	80	90	100
ISKSTGQEYA	AKFLKKRRRG	QDCRAEILHE	IAVLELAKSC	PRVINLHEVY
110	120	130	140	150
ENTSEIILIL	EYAAGGEIFS	LCLPELAEMV	SENDVIRLIK	QILEGVYYLH
160	170	180	190	200
QNNIVHLDLK	PQNILLSSIY	PLGDIKIVDF	GMSRKIGHAC	ELREIMGTPE
210	220	230	240	250
YLAPEILNYD	PITTATDMWN	IGIIAYMLLT	HTSPFVGEDN	QETYLNISQV
260	270	280	290	300
NVDYSEETFS	SVSQLATDFI	QSLLVKNPEK	RPTAEICLSH	SWLQQWDFEN
310	320	330	340	350
LFHPEETSSS	SQTQDHSVRS	SEDKTSKSSC	NGTCGDREDK	ENIPEDSSMV
360	370	372
SKRFRFDDSL	PNPHELVSDL	LC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1034 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK17B
Peptide Modified Sequence: SIS[+80.0]GLLTTTPQIPIK
Modification Type: Phospho (ST)
Protein - Site of Modification: 12
Peptide - Site of Modification: 3
Peptide Start: 10
Peptide End: 24

CPTAC ID: CPTAC-1034
Peptide Molecular Mass: 1,647.8848
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	34.5	10.5	19.2	42.7	42.1	14	54.9	43.4	23.8	13	21	14
y9 (1+)	12.5	12.1	12.5	34.8	44	12.8	37	45.6	17.9	13	21	14
y10 (1+)	22.6	11.2	12.1	36.5	42.4	16.7	42.9	43.9	20.6	13	21	14
sum	21	10.3	12.2	35.5	42.6	12.7	41.2	43.8	17.6	13	21	14

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3107 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK17B
Peptide Sequence: SISGLLTTTPQIPIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 10
Peptide End: 24

CPTAC ID: non-CPTAC-3107
Peptide Molecular Mass: 1,567.9185
Species: Homo Sapiens
Assay Type: Direct MRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend