IKBKG, CPTAC-1044 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
IKBKG	FIP3, NEMO

Sequence Length (AA)	Molecular Weight (Da)
419	48198

Protein Name
NF-kappa-B essential modulator

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MNRHLWKSQL	CEMVQPSGGP	AADQDVLGEE	SPLGKPAMLH	LPSEQGAPET
60	70	80	90	100
LQRCLEENQE	LRDAIRQSNQ	ILRERCEELL	HFQASQREEK	EFLMCKFQEA
110	120	130	140	150
RKLVERLGLE	KLDLKRQKEQ	ALREVEHLKR	CQQQMAEDKA	SVKAQVTSLL
160	170	180	190	200
GELQESQSRL	EAATKECQAL	EGRARAASEQ	ARQLESEREA	LQQQHSVQVD
210	220	230	240	250
QLRMQGQSVE	AALRMERQAA	SEEKRKLAQL	QVAYHQLFQE	YDNHIKSSVV
260	270	280	290	300
GSERKRGMQL	EDLKQQLQQA	EEALVAKQEV	IDKLKEEAEQ	HKIVMETVPV
310	320	330	340	350
LKAQADIYKA	DFQAERQARE	KLAEKKELLQ	EQLEQLQREY	SKLKASCQES
360	370	380	390	400
ARIEDMRKRH	VEVSQAPLPP	APAYLSSPLA	LPSQRRSPPE	EPPDFCCPKC
410	419
QYQAPDMDTL	QIHVMECIE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1044 Collapse assay details

Data source: Panorama

Official Gene Symbol: IKBKG
Peptide Modified Sequence: EC[+57.0]QALEGR
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: 167
Peptide - Site of Modification: 2
Peptide Start: 166
Peptide End: 173

CPTAC ID: CPTAC-1044
Peptide Molecular Mass: 961.4287
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	6.6	5.1	5.3	10.2	5	5.5	12.1	7.1	7.6	15	15	15
y5 (1+)	11.2	3.8	3.9	11.5	4.2	3.8	16.1	5.7	5.4	15	15	15
y4 (1+)	6.6	5.5	5.9	8.5	6.9	5.6	10.8	8.8	8.1	15	15	15
y3 (1+)	9.7	6.1	3.3	7.9	6.4	4.7	12.5	8.8	5.7	15	15	15
sum	5.9	2.2	2.7	6	2.9	3.2	8.4	3.6	4.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-806 Collapse assay details

Data source: Panorama

Official Gene Symbol: IKBKG
Peptide Sequence: ELLQEQLEQLQR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 327
Peptide End: 338

CPTAC ID: CPTAC-806
Peptide Molecular Mass: 1,525.8100
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend