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Overview Data source: UniProt

Official Gene Symbol Other Aliases
IKBKG FIP3,NEMO
Sequence Length (AA) Molecular Weight (Da)
419 48198
Protein Name
NF-kappa-B essential modulator
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MNRHLWKSQL CEMVQPSGGP AADQDVLGEE SPLGKPAMLH LPSEQGAPET
60 70 80 90 100
LQRCLEENQE LRDAIRQSNQ ILRERCEELL HFQASQREEK EFLMCKFQEA
110 120 130 140 150
RKLVERLGLE KLDLKRQKEQ ALREVEHLKR CQQQMAEDKA SVKAQVTSLL
160 170 180 190 200
GELQESQSRL EAATKECQAL EGRARAASEQ ARQLESEREA LQQQHSVQVD
210 220 230 240 250
QLRMQGQSVE AALRMERQAA SEEKRKLAQL QVAYHQLFQE YDNHIKSSVV
260 270 280 290 300
GSERKRGMQL EDLKQQLQQA EEALVAKQEV IDKLKEEAEQ HKIVMETVPV
310 320 330 340 350
LKAQADIYKA DFQAERQARE KLAEKKELLQ EQLEQLQREY SKLKASCQES
360 370 380 390 400
ARIEDMRKRH VEVSQAPLPP APAYLSSPLA LPSQRRSPPE EPPDFCCPKC
410 419
QYQAPDMDTL QIHVMECIE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1044 Collapse assay details

Data source: Panorama

Official Gene Symbol
IKBKG
Peptide Modified Sequence
EC[+57.0]QALEGR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
167
Peptide - Site of Modification
2
Peptide Start
166
Peptide End
173
CPTAC ID
CPTAC-1044
Peptide Molecular Mass
961.4287
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6.6 5.1 5.3 10.2 5 5.5 12.1 7.1 7.6 15 15 15
y5 (1+) 11.2 3.8 3.9 11.5 4.2 3.8 16.1 5.7 5.4 15 15 15
y4 (1+) 6.6 5.5 5.9 8.5 6.9 5.6 10.8 8.8 8.1 15 15 15
y3 (1+) 9.7 6.1 3.3 7.9 6.4 4.7 12.5 8.8 5.7 15 15 15
sum 5.9 2.2 2.7 6 2.9 3.2 8.4 3.6 4.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-806 Collapse assay details

Data source: Panorama

Official Gene Symbol
IKBKG
Peptide Sequence
ELLQEQLEQLQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
327
Peptide End
338
CPTAC ID
CPTAC-806
Peptide Molecular Mass
1,525.8100
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 10.4 17.2 10.3 24.8 16.8 13.8 26.9 24 17.2 15 15 15
y4 (1+) 9.1 9.9 7.4 9.7 10.1 10 13.3 14.1 12.4 15 15 15
y5 (1+) 14.6 9.7 7 15.7 9.6 7.2 21.4 13.6 10 15 15 15
y6 (1+) 14.8 7.9 6.8 15.9 9.1 8.6 21.7 12.1 11 15 15 15
y7 (1+) 10.5 10 5.8 15.8 10.2 10.1 19 14.3 11.6 15 15 15
y8 (1+) 7.2 6.7 7.3 9 10.3 7.1 11.5 12.3 10.2 15 15 15
y9 (1+) 13.2 6.5 5.6 15.2 8.3 7.2 20.1 10.5 9.1 15 15 15
y10 (1+) 25.2 8.8 11.2 26.2 9.7 14.8 36.4 13.1 18.6 15 15 15
sum 5.2 3.3 3.3 9 5.4 5.3 10.4 6.3 6.2 15 15 15

Additional Resources and Comments