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Overview Data source: UniProt

Official Gene Symbol Other Aliases
JUN N/A
Sequence Length (AA) Molecular Weight (Da)
331 35676
Protein Name
Transcription factor AP-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTAKMETTFY DDALNASFLP SESGPYGYSN PKILKQSMTL NLADPVGSLK
60 70 80 90 100
PHLRAKNSDL LTSPDVGLLK LASPELERLI IQSSNGHITT TPTPTQFLCP
110 120 130 140 150
KNVTDEQEGF AEGFVRALAE LHSQNTLPSV TSAAQPVNGA GMVAPAVASV
160 170 180 190 200
AGGSGSGGFS ASLHSEPPVY ANLSNFNPGA LSSGGGAPSY GAAGLAFPAQ
210 220 230 240 250
PQQQQQPPHH LPQQMPVQHP RLQALKEEPQ TVPEMPGETP PLSPIDMESQ
260 270 280 290 300
ERIKAERKRM RNRIAASKCR KRKLERIARL EEKVKTLKAQ NSELASTANM
310 320 330 331
LREQVAQLKQ KVMNHVNSGC QLMLTQQLQT F

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1324 Collapse assay details

Data source: Panorama

Official Gene Symbol
JUN
Peptide Modified Sequence
NSDLLTS[+80.0]PDVGLLK
Modification Type
Phospho (ST)
Protein - Site of Modification
63
Peptide - Site of Modification
7
Peptide Start
57
Peptide End
70
CPTAC ID
CPTAC-1324
Peptide Molecular Mass
1,550.7593
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Digested Cell Lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 3 um, 200A
Column Dimensions
0.075 x 100 mm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 14 17 13.3 19.2 16.1 19.1 23.8 23.4 23.3 15 15 15
y9 (1+) 17.8 21.6 9.6 18.1 29.1 13 25.4 36.2 16.2 15 15 15
y7 (1+) 20 11.1 8.4 23.9 12.6 13.8 31.2 16.8 16.2 15 15 15
sum 11 6.4 6.4 13.6 11.2 11.7 17.5 12.9 13.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-927 Collapse assay details

Data source: Panorama

Official Gene Symbol
JUN
Peptide Modified Sequence
NSDLLTS[+80.0]PDVGLLK
Modification Type
Phospho (ST)
Protein - Site of Modification
63
Peptide - Site of Modification
7
Peptide Start
57
Peptide End
70
CPTAC ID
CPTAC-927
Peptide Molecular Mass
1,550.7593
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 70.2 16.5 11.5 63.3 19.1 18.1 94.5 25.2 21.4 15 24 15
y8 (1+) 41.2 22 15.9 52.4 24.9 23.2 66.7 33.2 28.1 15 24 15
y9 (1+) 51.9 14.5 15.8 42.5 21.3 15.4 67.1 25.8 22.1 15 24 15
sum 45.8 14 12.2 41.4 18.5 13.1 61.7 23.2 17.9 15 24 15

Additional Resources and Comments


Assay Details for CPTAC-3232 Collapse assay details

Data source: Panorama

Official Gene Symbol
JUN
Peptide Sequence
NSDLLTSPDVGLLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
57
Peptide End
70
CPTAC ID
CPTAC-3232
Peptide Molecular Mass
1,470.7930
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

View Details (opens in a new window)

Targeted mass spectrometry enables robust quantification of FANCD2 mono-ubiquitination in response to DNA damage. Whiteaker JR, Zhao L, Ivey RG, Sanchez-Bonilla M, Moore HD, Schoenherr RM, Yan P, Lin C, Shimamura A, Paulovich AG. DNA Repair (Amst). 2018 May;65:47-53. doi: 10.1016/j.dnarep.2018.03.003. Epub 2018 Mar 21.

View Details (opens in a new window)

Targeted mass spectrometry enables robust quantification of FANCD2 mono-ubiquitination in response to DNA damage. Whiteaker JR, Zhao L, Ivey RG, Sanchez-Bonilla M, Moore HD, Schoenherr RM, Yan P, Lin C, Shimamura A, Paulovich AG. DNA Repair (Amst). 2018 May;65:47-53. doi: 10.1016/j.dnarep.2018.03.003. Epub 2018 Mar 21.

View Details (opens in a new window)

None


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 18.1 14.6 16 20.2 14.3 20.9 27.1 20.4 26.3 15 15 15
y8 (1+) 13.8 16.5 12.6 19.8 25.3 20.3 24.1 30.2 23.9 15 15 15
y9 (1+) 12.6 9.8 21.3 15.9 10.4 30.4 20.3 14.3 37.1 15 15 15
sum 11.5 6.7 15.6 14 8.7 23.9 18.1 11 28.5 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-3233 Collapse assay details

Data source: Panorama

Official Gene Symbol
JUN
Peptide Modified Sequence
NSDLLTS[+80.0]PDVGLLK
Modification Type
Phospho (ST)
Protein - Site of Modification
63
Peptide - Site of Modification
7
Peptide Start
57
Peptide End
70
CPTAC ID
CPTAC-3233
Peptide Molecular Mass
1,550.7593
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

View Details (opens in a new window)

Targeted mass spectrometry enables robust quantification of FANCD2 mono-ubiquitination in response to DNA damage. Whiteaker JR, Zhao L, Ivey RG, Sanchez-Bonilla M, Moore HD, Schoenherr RM, Yan P, Lin C, Shimamura A, Paulovich AG. DNA Repair (Amst). 2018 May;65:47-53. doi: 10.1016/j.dnarep.2018.03.003. Epub 2018 Mar 21.

View Details (opens in a new window)

Targeted mass spectrometry enables robust quantification of FANCD2 mono-ubiquitination in response to DNA damage. Whiteaker JR, Zhao L, Ivey RG, Sanchez-Bonilla M, Moore HD, Schoenherr RM, Yan P, Lin C, Shimamura A, Paulovich AG. DNA Repair (Amst). 2018 May;65:47-53. doi: 10.1016/j.dnarep.2018.03.003. Epub 2018 Mar 21.

View Details (opens in a new window)

None


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 17.5 10.6 11.9 32.7 18.6 16.3 37.1 21.4 20.2 15 15 15
y8 (1+) 32.1 11.9 7.4 30.2 25.9 14.8 44.1 28.5 16.5 15 15 15
y9 (1+) 23.1 6.7 13.1 28.1 14.8 14.9 36.4 16.2 19.8 15 15 15
y9-98 (1+) 38.4 17 10.8 55.6 19.6 16.4 67.6 25.9 19.6 15 15 15
sum 13.6 5.6 7.8 20.1 16.4 13 24.3 17.3 15.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1222 Collapse assay details

Data source: Panorama

Official Gene Symbol
JUN
Peptide Sequence
NSDLLTSPDVGLLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
57
Peptide End
70
CPTAC ID
CPTAC-1222
Peptide Molecular Mass
1,470.7930
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 32.6 6.6 7.4 34.4 11.6 13 47.4 13.3 15 15 15 15
y7 (1+) 9 5.7 5.1 19.5 13.3 11.5 21.5 14.5 12.6 15 15 15
y9 (1+) 7.7 4.5 5.4 12.2 10 11.9 14.4 11 13.1 15 15 15
sum 8 4.9 5.2 13.7 11.5 11.7 15.9 12.5 12.8 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-807 Collapse assay details

Data source: Panorama

Official Gene Symbol
JUN
Peptide Modified Sequence
LAS[+80.0]PELER
Modification Type
Phospho (ST)
Protein - Site of Modification
73
Peptide - Site of Modification
3
Peptide Start
71
Peptide End
78
CPTAC ID
CPTAC-807
Peptide Molecular Mass
993.4532
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 12.9 3.5 3.3 13.1 4.3 3.9 18.4 5.5 5.1 15 15 15
y4 (1+) 7.7 4.2 4.4 10.8 4 3.9 13.3 5.8 5.9 15 15 15
y5 (1+) 8 2.3 3.1 9.5 2.9 3.7 12.4 3.7 4.8 15 15 15
b3 (1+) 23.9 11.4 8.9 20 11.3 7.9 31.2 16.1 11.9 15 15 15
sum 6.6 2 2.2 6.7 2.3 2.8 9.4 3 3.6 15 15 15

Additional Resources and Comments