JUN, CPTAC-6257 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
JUN	N/A

Sequence Length (AA)	Molecular Weight (Da)
331	35676

Protein Name
Transcription factor Jun

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTAKMETTFY	DDALNASFLP	SESGPYGYSN	PKILKQSMTL	NLADPVGSLK
60	70	80	90	100
PHLRAKNSDL	LTSPDVGLLK	LASPELERLI	IQSSNGHITT	TPTPTQFLCP
110	120	130	140	150
KNVTDEQEGF	AEGFVRALAE	LHSQNTLPSV	TSAAQPVNGA	GMVAPAVASV
160	170	180	190	200
AGGSGSGGFS	ASLHSEPPVY	ANLSNFNPGA	LSSGGGAPSY	GAAGLAFPAQ
210	220	230	240	250
PQQQQQPPHH	LPQQMPVQHP	RLQALKEEPQ	TVPEMPGETP	PLSPIDMESQ
260	270	280	290	300
ERIKAERKRM	RNRIAASKCR	KRKLERIARL	EEKVKTLKAQ	NSELASTANM
310	320	330	331
LREQVAQLKQ	KVMNHVNSGC	QLMLTQQLQT	F

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1324 Collapse assay details

Data source: Panorama

Official Gene Symbol: JUN
Peptide Modified Sequence: NSDLLTS[+80.0]PDVGLLK
Modification Type: Phospho (ST)
Protein - Site of Modification: 63
Peptide - Site of Modification: 7
Peptide Start: 57
Peptide End: 70

CPTAC ID: CPTAC-1324
Peptide Molecular Mass: 1,550.7593
Species: Homo sapiens (Human)
Assay Type: Enrichment PRM
Enrichment Method: immunoaffinity enrichment
Matrix: Digested Cell Lysate
Submitting Laboratory: Broad Institute
Submitting Lab PI: Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Quantiva TSQ
Internal Standard: medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy NanoLC1000
Column Packing: Reprosil C18, 3 um, 200A
Column Dimensions: 0.075 x 100 mm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	14	17	13.3	19.2	16.1	19.1	23.8	23.4	23.3	15	15	15
y9 (1+)	17.8	21.6	9.6	18.1	29.1	13	25.4	36.2	16.2	15	15	15
y7 (1+)	20	11.1	8.4	23.9	12.6	13.8	31.2	16.8	16.2	15	15	15
sum	11	6.4	6.4	13.6	11.2	11.7	17.5	12.9	13.3	15	15	15

Additional Resources and Comments

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Comments

Assay Details for non-CPTAC-6257 Collapse assay details

Data source: Panorama

Official Gene Symbol: JUN
Peptide Modified Sequence: LAS[+79.966331]PELER
Modification Type: Phospho (ST)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 8
Peptide Start: 71
Peptide End: 78

CPTAC ID: non-CPTAC-6257
Peptide Molecular Mass: 993.4532
Species: Human
Assay Type: Enrichment PRM
Enrichment Method: immunoaffinity enrichment
Matrix: SKBR3 cells
Submitting Laboratory: Cell Signaling Technology
Submitting Lab PI: Yiying Zhu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Orbitrap Q Exactive (Thermo)
Internal Standard: Heavy isotope-labeled peptides
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N

LC: EASY-nLC II (Thermo)
Column Packing: self-packed
Column Dimensions: ID 100um, length 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	4.2	6.1	12.3	4.7	7.9	11.1	6.3	10	16.6	15	15	15
y7-98 (1+)	4	6.4	11.8	4.7	8.1	10.5	6.2	10.3	15.8	15	15	15
y6-98 (1+)	5.5	7.3	11.5	5.4	7.9	10.1	7.7	10.8	15.3	15	15	15
y6-98 (2+)	4	5.9	10.8	4.1	6.8	9.5	5.7	9	14.4	15	15	15
y7-98 (2+)	2.9	7.9	11.6	3.3	8.8	10	4.4	11.8	15.3	15	15	15
sum	3.6	6.4	11.4	3.6	7.5	10.1	5.1	9.9	15.2	15	15	15

Additional Resources and Comments

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Comments

Assay Details for CPTAC-927 Collapse assay details

Data source: Panorama

Official Gene Symbol: JUN
Peptide Modified Sequence: NSDLLTS[+80.0]PDVGLLK
Modification Type: Phospho (ST)
Protein - Site of Modification: 63
Peptide - Site of Modification: 7
Peptide Start: 57
Peptide End: 70

CPTAC ID: CPTAC-927
Peptide Molecular Mass: 1,550.7593
Species: Homo sapiens (Human)
Assay Type: Enrichment PRM
Enrichment Method: immunoaffinity enrichment
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTrap 5500 (Sciex)
Internal Standard: Peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-2D (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 75 µm x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	70.2	16.5	11.5	63.3	19.1	18.1	94.5	25.2	21.4	15	24	15
y8 (1+)	41.2	22	15.9	52.4	24.9	23.2	66.7	33.2	28.1	15	24	15
y9 (1+)	51.9	14.5	15.8	42.5	21.3	15.4	67.1	25.8	22.1	15	24	15
sum	45.8	14	12.2	41.4	18.5	13.1	61.7	23.2	17.9	15	24	15

Additional Resources and Comments

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Comments

Assay Details for CPTAC-3232 Collapse assay details

Data source: Panorama

Official Gene Symbol: JUN
Peptide Sequence: NSDLLTSPDVGLLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 57
Peptide End: 70

CPTAC ID: CPTAC-3232
Peptide Molecular Mass: 1,470.7930
Species: Homo sapiens (Human)
Assay Type: Enrichment PRM
Enrichment Method: immunoaffinity enrichment
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6500 QTRAP (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Ultra NanoLC 2D+ (Eksigent)
Column Packing: ReproSil-Pur C18-AQ 3um
Column Dimensions: 100 x 0.075 mm
Flow Rate: 300 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	18.1	14.6	16	20.2	14.3	20.9	27.1	20.4	26.3	15	15	15
y8 (1+)	13.8	16.5	12.6	19.8	25.3	20.3	24.1	30.2	23.9	15	15	15
y9 (1+)	12.6	9.8	21.3	15.9	10.4	30.4	20.3	14.3	37.1	15	15	15
sum	11.5	6.7	15.6	14	8.7	23.9	18.1	11	28.5	15	15	15

Additional Resources and Comments

Resources

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Comments

Assay Details for CPTAC-3233 Collapse assay details

Data source: Panorama

Official Gene Symbol: JUN
Peptide Modified Sequence: NSDLLTS[+80.0]PDVGLLK
Modification Type: Phospho (ST)
Protein - Site of Modification: 63
Peptide - Site of Modification: 7
Peptide Start: 57
Peptide End: 70

CPTAC ID: CPTAC-3233
Peptide Molecular Mass: 1,550.7593
Species: Homo sapiens (Human)
Assay Type: Enrichment PRM
Enrichment Method: immunoaffinity enrichment
Matrix: cell lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6500 QTRAP (Sciex)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Ultra NanoLC 2D+ (Eksigent)
Column Packing: ReproSil-Pur C18-AQ 3um
Column Dimensions: 100 x 0.075 mm
Flow Rate: 300 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	17.5	10.6	11.9	32.7	18.6	16.3	37.1	21.4	20.2	15	15	15
y8 (1+)	32.1	11.9	7.4	30.2	25.9	14.8	44.1	28.5	16.5	15	15	15
y9 (1+)	23.1	6.7	13.1	28.1	14.8	14.9	36.4	16.2	19.8	15	15	15
y9-98 (1+)	38.4	17	10.8	55.6	19.6	16.4	67.6	25.9	19.6	15	15	15
sum	13.6	5.6	7.8	20.1	16.4	13	24.3	17.3	15.2	15	15	15

Additional Resources and Comments

Resources

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Comments

Assay Details for CPTAC-1222 Collapse assay details

Data source: Panorama

Official Gene Symbol: JUN
Peptide Sequence: NSDLLTSPDVGLLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 57
Peptide End: 70

CPTAC ID: CPTAC-1222
Peptide Molecular Mass: 1,470.7930
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	32.6	6.6	7.4	34.4	11.6	13	47.4	13.3	15	15	15	15
y7 (1+)	9	5.7	5.1	19.5	13.3	11.5	21.5	14.5	12.6	15	15	15
y9 (1+)	7.7	4.5	5.4	12.2	10	11.9	14.4	11	13.1	15	15	15
sum	8	4.9	5.2	13.7	11.5	11.7	15.9	12.5	12.8	15	15	15

Additional Resources and Comments

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Comments

Assay Details for CPTAC-807 Collapse assay details

Data source: Panorama

Official Gene Symbol: JUN
Peptide Modified Sequence: LAS[+80.0]PELER
Modification Type: Phospho (ST)
Protein - Site of Modification: 73
Peptide - Site of Modification: 3
Peptide Start: 71
Peptide End: 78

CPTAC ID: CPTAC-807
Peptide Molecular Mass: 993.4532
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	12.9	3.5	3.3	13.1	4.3	3.9	18.4	5.5	5.1	15	15	15
y4 (1+)	7.7	4.2	4.4	10.8	4	3.9	13.3	5.8	5.9	15	15	15
y5 (1+)	8	2.3	3.1	9.5	2.9	3.7	12.4	3.7	4.8	15	15	15
b3 (1+)	23.9	11.4	8.9	20	11.3	7.9	31.2	16.1	11.9	15	15	15
sum	6.6	2	2.2	6.7	2.3	2.8	9.4	3	3.6	15	15	15

Search form

JUN

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1324 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for non-CPTAC-6257 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for CPTAC-927 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for CPTAC-3232 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

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Assay Details for CPTAC-3233 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

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Repeatability

Additional Resources and Comments

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Assay Details for CPTAC-1222 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for CPTAC-807 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments