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Overview Data source: UniProt

Official Gene Symbol Other Aliases
PRDX6 AOP2,KIAA0106
Sequence Length (AA) Molecular Weight (Da)
224 25035
Protein Name
Peroxiredoxin-6
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE
60 70 80 90 100
LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF
110 120 130 140 150
PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP
160 170 180 190 200
ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK
210 220 224
LFPKGVFTKE LPSGKKYLRY TPQP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-117 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRDX6
Peptide Modified Sequence
DFTPVC[+57.0]TTELGR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
47
Peptide - Site of Modification
6
Peptide Start
42
Peptide End
53
CPTAC ID
CPTAC-117
Peptide Molecular Mass
1,394.6500
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 14.5 5.8 7.7 14.6 7.1 12.6 20.6 9.2 14.8 15 25 15
y8 (1+) 27 7.7 6.9 28.2 10.9 8.6 39 13.3 11 15 25 15
y7 (1+) 9.8 6.4 6.7 11.4 6.5 12.4 15 9.1 14.1 15 25 15
sum 10.1 5.4 7 13 6.2 11.7 16.5 8.2 13.6 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-118 Collapse assay details

Data source: Panorama

Official Gene Symbol
PRDX6
Peptide Sequence
LPFPIIDDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
98
Peptide End
106
CPTAC ID
CPTAC-118
Peptide Molecular Mass
1,084.5917
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 17.5 5.6 4.8 19.6 13.1 11.2 26.3 14.2 12.2 15 25 15
y6 (1+) 17.3 4.9 3.8 18.3 11 11 25.2 12 11.6 15 25 15
y5 (1+) 35.1 7.5 7.4 37.7 11.1 12.7 51.5 13.4 14.7 15 25 15
sum 17 4.7 3.5 17.1 11.2 10.4 24.1 12.1 11 15 25 15

Additional Resources and Comments


Assay Details for non-CPTAC-3603 Collapse assay details

Data source: Panorama

Official Gene Symbol
Prdx6
Peptide Sequence
LSILYPATTGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
145
Peptide End
155
CPTAC ID
non-CPTAC-3603
Peptide Molecular Mass
1,190.6659
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 13.8 4.3 3.7 19.3 19.9 17.6 23.7 20.4 18 15 15 15
b3 (1+) 20.9 4.5 2 48 8.2 3.7 52.4 9.4 4.2 15 15 15
y6 (1+) 6 2.9 2.2 13.6 8.9 5.4 14.9 9.4 5.8 15 15 15
y7 (1+) 15.6 7.1 3.1 37.2 36.5 30.8 40.3 37.2 31 15 15 15
y8 (1+) 11.7 5 2.4 15.1 8.8 6.4 19.1 10.1 6.8 15 15 15
sum 4.1 2.5 1.5 6.5 15.5 11.3 7.7 15.7 11.4 15 15 15

Additional Resources and Comments