YWHAB, CPTAC-142 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
YWHAB	N/A

Sequence Length (AA)	Molecular Weight (Da)
246	28082

Protein Name
14-3-3 protein beta/alpha

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTMDKSELVQ	KAKLAEQAER	YDDMAAAMKA	VTEQGHELSN	EERNLLSVAY
60	70	80	90	100
KNVVGARRSS	WRVISSIEQK	TERNEKKQQM	GKEYREKIEA	ELQDICNDVL
110	120	130	140	150
ELLDKYLIPN	ATQPESKVFY	LKMKGDYFRY	LSEVASGDNK	QTTVSNSQQA
160	170	180	190	200
YQEAFEISKK	EMQPTHPIRL	GLALNFSVFY	YEILNSPEKA	CSLAKTAFDE
210	220	230	240	246
AIAELDTLNE	ESYKDSTLIM	QLLRDNLTLW	TSENQGDEGD	AGEGEN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-142 Collapse assay details

Data source: Panorama

Official Gene Symbol: YWHAB
Peptide Sequence: YLIPNATQPESK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 106
Peptide End: 117

CPTAC ID: CPTAC-142
Peptide Molecular Mass: 1,359.7034
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	9.3	8.4	3.9	18.2	11.8	9.5	20.4	14.5	10.3	15	25	15
y8 (1+)	21.8	10.5	4.9	38.9	15.2	7.4	44.6	18.5	8.9	15	25	15
y6 (1+)	33.1	11.8	8.7	35.2	14.9	9.5	48.3	19	12.9	15	25	15
sum	13.1	8.2	3.9	26.2	12	9.1	29.3	14.5	9.9	15	25	15

YWHAB

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-142 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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YWHAB

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-142 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments