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Overview Data source: UniProt

Official Gene Symbol Other Aliases
YWHAQ N/A
Sequence Length (AA) Molecular Weight (Da)
245 27764
Protein Name
14-3-3 protein theta
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN
60 70 80 90 100
VVGGRRSAWR VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL
110 120 130 140 150
LDKYLIANAT NPESKVFYLK MKGDYFRYLA EVACGDDRKQ TIDNSQGAYQ
160 170 180 190 200
EAFDISKKEM QPTHPIRLGL ALNFSVFYYE ILNNPELACT LAKTAFDEAI
210 220 230 240 245
AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-144 Collapse assay details

Data source: Panorama

Official Gene Symbol
YWHAQ
Peptide Modified Sequence
YLAEVAC[+57.0]GDDR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
134
Peptide - Site of Modification
7
Peptide Start
128
Peptide End
138
CPTAC ID
CPTAC-144
Peptide Molecular Mass
1,267.5503
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 24 11.5 12.1 29.5 11.3 19.6 38 16.1 23 15 25 15
y7 (1+) 24.9 8.3 13.4 35.8 8.8 18.8 43.6 12.1 23.1 15 25 15
y6 (1+) 27.4 9.2 9.8 30.6 9.2 15.4 41.1 13 18.3 15 25 15
sum 24 9.4 11.2 30 9.4 17.5 38.4 13.3 20.8 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-143 Collapse assay details

Data source: Panorama

Official Gene Symbol
YWHAQ
Peptide Sequence
YLIANATNPESK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
104
Peptide End
115
CPTAC ID
CPTAC-143
Peptide Molecular Mass
1,319.6721
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 23.4 8.1 6.3 29.1 12.3 10.3 37.3 14.7 12.1 15 25 15
y9 (1+) 15.1 8.9 6.4 16.4 12.5 10.1 22.3 15.3 12 15 25 15
y8 (1+) 16.9 7.9 3.6 22.6 10.5 12.8 28.2 13.1 13.3 15 25 15
sum 11.8 8 2.9 17 11.3 9 20.7 13.8 9.5 15 25 15

Additional Resources and Comments


Assay Details for non-CPTAC-3627 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ywhaq
Peptide Sequence
AVTEQGAELSNEER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
28
Peptide End
41
CPTAC ID
non-CPTAC-3627
Peptide Molecular Mass
1,531.7114
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 16.1 7.9 4.1 12.4 6.5 7 20.3 10.2 8.1 15 15 15
y9 (1+) 6.4 5.9 3.5 8.3 6.5 5.8 10.5 8.8 6.8 15 15 15
y12 (2+) 15.8 14.9 8.9 20.8 14.5 9.6 26.1 20.8 13.1 15 15 15
y5 (1+) 13.5 7.1 4.9 16.4 6.7 7.5 21.2 9.8 9 15 15 15
y6 (1+) 12.7 4.1 5.4 13.3 6.5 8.5 18.4 7.7 10.1 15 15 15
sum 3.6 2.7 1.3 2.6 2.9 4.4 4.4 4 4.6 15 15 15

Additional Resources and Comments