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Overview Data source: UniProt

Official Gene Symbol Other Aliases
ALDH7A1 ATQ1
Sequence Length (AA) Molecular Weight (Da)
539 58487
Protein Name
Alpha-aminoadipic semialdehyde dehydrogenase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWRLPRALCV HAAKTSKLSG PWSRPAAFMS TLLINQPQYA WLKELGLREE
60 70 80 90 100
NEGVYNGSWG GRGEVITTYC PANNEPIARV RQASVADYEE TVKKAREAWK
110 120 130 140 150
IWADIPAPKR GEIVRQIGDA LREKIQVLGS LVSLEMGKIL VEGVGEVQEY
160 170 180 190 200
VDICDYAVGL SRMIGGPILP SERSGHALIE QWNPVGLVGI ITAFNFPVAV
210 220 230 240 250
YGWNNAIAMI CGNVCLWKGA PTTSLISVAV TKIIAKVLED NKLPGAICSL
260 270 280 290 300
TCGGADIGTA MAKDERVNLL SFTGSTQVGK QVGLMVQERF GRSLLELGGN
310 320 330 340 350
NAIIAFEDAD LSLVVPSALF AAVGTAGQRC TTARRLFIHE SIHDEVVNRL
360 370 380 390 400
KKAYAQIRVG NPWDPNVLYG PLHTKQAVSM FLGAVEEAKK EGGTVVYGGK
410 420 430 440 450
VMDRPGNYVE PTIVTGLGHD ASIAHTETFA PILYVFKFKN EEEVFAWNNE
460 470 480 490 500
VKQGLSSSIF TKDLGRIFRW LGPKGSDCGI VNVNIPTSGA EIGGAFGGEK
510 520 530 539
HTGGGRESGS DAWKQYMRRS TCTINYSKDL PLAQGIKFQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-15 Collapse assay details

Data source: Panorama

Official Gene Symbol
ALDH7A1
Peptide Sequence
GAPTTSLISVAVTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
219
Peptide End
232
CPTAC ID
CPTAC-15
Peptide Molecular Mass
1,343.7660
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 7.5 9.1 15.2 9.9 11.8 14.1 12.4 14.9 20.7 25 15 15
y8 (1+) 9.8 11.3 15.2 12.8 18.6 16.3 16.1 21.8 22.3 25 15 15
y7 (1+) 7.1 8.9 6.1 12.8 10.2 11 14.6 13.5 12.6 25 15 15
sum 6.5 8.1 5 10.5 9.9 7.9 12.3 12.8 9.3 25 15 15

Additional Resources and Comments


Assay Details for CPTAC-16 Collapse assay details

Data source: Panorama

Official Gene Symbol
ALDH7A1
Peptide Sequence
VNLLSFTGSTQVGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
267
Peptide End
280
CPTAC ID
CPTAC-16
Peptide Molecular Mass
1,449.7827
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 26.4 8.8 8.1 32.9 12.1 15.6 42.2 15 17.6 15 25 15
y10 (1+) 16.9 5.4 5.6 19.7 10.6 12 26 11.9 13.2 15 25 15
y8 (1+) 44.3 7.8 8.5 41.1 12.6 15.2 60.4 14.8 17.4 15 25 15
sum 22.9 5.1 5.2 23.2 10.4 12.7 32.6 11.6 13.7 15 25 15

Additional Resources and Comments