ALDH2, CPTAC-14 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ALDH2	ALDM

Sequence Length (AA)	Molecular Weight (Da)
517	56381

Protein Name
Aldehyde dehydrogenase, mitochondrial

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLRAAARFGP	RLGRRLLSAA	ATQAVPAPNQ	QPEVFCNQIF	INNEWHDAVS
60	70	80	90	100
RKTFPTVNPS	TGEVICQVAE	GDKEDVDKAV	KAARAAFQLG	SPWRRMDASH
110	120	130	140	150
RGRLLNRLAD	LIERDRTYLA	ALETLDNGKP	YVISYLVDLD	MVLKCLRYYA
160	170	180	190	200
GWADKYHGKT	IPIDGDFFSY	TRHEPVGVCG	QIIPWNFPLL	MQAWKLGPAL
210	220	230	240	250
ATGNVVVMKV	AEQTPLTALY	VANLIKEAGF	PPGVVNIVPG	FGPTAGAAIA
260	270	280	290	300
SHEDVDKVAF	TGSTEIGRVI	QVAAGSSNLK	RVTLELGGKS	PNIIMSDADM
310	320	330	340	350
DWAVEQAHFA	LFFNQGQCCC	AGSRTFVQED	IYDEFVERSV	ARAKSRVVGN
360	370	380	390	400
PFDSKTEQGP	QVDETQFKKI	LGYINTGKQE	GAKLLCGGGI	AADRGYFIQP
410	420	430	440	450
TVFGDVQDGM	TIAKEEIFGP	VMQILKFKTI	EEVVGRANNS	TYGLAAAVFT
460	470	480	490	500
KDLDKANYLS	QALQAGTVWV	NCYDVFGAQS	PFGGYKMSGS	GRELGEYGLQ
510	517
AYTEVKTVTV	KVPQKNS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-12 Collapse assay details

Data source: Panorama

Official Gene Symbol: ALDH2
Peptide Sequence: VAEQTPLTALYVANLIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 210
Peptide End: 226

CPTAC ID: CPTAC-12
Peptide Molecular Mass: 1,843.0455
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	12.4	8.6	14.4	20.7	10.7	15.2	24.1	13.7	20.9	25	15	15
y9 (1+)	15.1	6.9	13.2	17.7	8.4	24.4	23.3	10.9	27.7	25	15	15
y7 (1+)	12	14.2	9.8	17.9	19.7	13.6	21.6	24.3	16.8	25	15	15
sum	9.5	6.8	8.3	16.7	11.1	14.2	19.2	13	16.4	25	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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