ACTR3, CPTAC-161 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ACTR3	ARP3

Sequence Length (AA)	Molecular Weight (Da)
418	47371

Protein Name
Actin-related protein 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGRLPACVV	DCGTGYTKLG	YAGNTEPQFI	IPSCIAIKES	AKVGDQAQRR
60	70	80	90	100
VMKGVDDLDF	FIGDEAIEKP	TYATKWPIRH	GIVEDWDLME	RFMEQVIFKY
110	120	130	140	150
LRAEPEDHYF	LLTEPPLNTP	ENREYTAEIM	FESFNVPGLY	IAVQAVLALA
160	170	180	190	200
ASWTSRQVGE	RTLTGTVIDS	GDGVTHVIPV	AEGYVIGSCI	KHIPIAGRDI
210	220	230	240	250
TYFIQQLLRD	REVGIPPEQS	LETAKAVKER	YSYVCPDLVK	EFNKYDTDGS
260	270	280	290	300
KWIKQYTGIN	AISKKEFSID	VGYERFLGPE	IFFHPEFANP	DFTQPISEVV
310	320	330	340	350
DEVIQNCPID	VRRPLYKNIV	LSGGSTMFRD	FGRRLQRDLK	RTVDARLKLS
360	370	380	390	400
EELSGGRLKP	KPIDVQVITH	HMQRYAVWFG	GSMLASTPEF	YQVCHTKKDY
410	418
EEIGPSICRH	NPVFGVMS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-161 Collapse assay details

Data source: Panorama

Official Gene Symbol: ACTR3
Peptide Sequence: AEPEDHYFLLTEPPLNTPENR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 103
Peptide End: 123

CPTAC ID: CPTAC-161
Peptide Molecular Mass: 2,481.1812
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	13.4	9.5	6.5	24.4	11.9	12.1	27.8	15.2	13.7	15	25	15
y8 (1+)	19.8	15.3	8.2	21.6	17.9	18.3	29.3	23.5	20.1	15	25	15
y19 (2+)	53.1	66.3	30.9	52.5	69.2	32.3	74.7	95.8	44.7	15	25	15
sum	9.4	11.2	6.5	20.7	13.1	13.3	22.7	17.2	14.8	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

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