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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ADK N/A
Sequence Length (AA) Molecular Weight (Da)
362 40545
Protein Name
Adenosine kinase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAAEEEPKP KKLKVEAPQA LRENILFGMG NPLLDISAVV DKDFLDKYSL
60 70 80 90 100
KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA
110 120 130 140 150
ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS
160 170 180 190 200
LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA
210 220 230 240 250
HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE
260 270 280 290 300
QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA
310 320 330 340 350
VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR
360 362
TGCTFPEKPD FH

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-162 Collapse assay details

Data source: Panorama

Official Gene Symbol
ADK
Peptide Modified Sequence
SLIANLAAANC[+57.0]YK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
160
Peptide - Site of Modification
11
Peptide Start
150
Peptide End
162
CPTAC ID
CPTAC-162
Peptide Molecular Mass
1,407.7180
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 13.6 13 12.1 12.4 9.5 15 18.4 16.1 19.3 15 25 15
y7 (1+) 6.1 7 8.4 11.7 10.9 10.8 13.2 13 13.7 15 25 15
y6 (1+) 8.4 7 9 12.3 9.1 12.1 14.9 11.5 15.1 15 25 15
sum 6.3 6.8 8.8 9.4 9 11.2 11.3 11.3 14.2 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-2926 Collapse assay details

Data source: Panorama

Official Gene Symbol
ADK
Peptide Sequence
IFTLNLSAPFISQFYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
209
Peptide End
224
CPTAC ID
CPTAC-2926
Peptide Molecular Mass
1,888.0135
Species
Homo Sapiens
Assay Type
Direct MRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 12.8 3.6 6.4 20.5 6.7 14.3 24.2 7.6 15.7 15 15 15
y8 (1+) 12.6 4 7.9 19.7 7.9 16.4 23.4 8.9 18.2 15 15 15
y10 (1+) 11.3 3.8 7.7 18 8.4 16.4 21.3 9.2 18.1 15 15 15
sum 12.1 3.8 7.5 19.2 7.8 15.9 22.7 8.7 17.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2925 Collapse assay details

Data source: Panorama

Official Gene Symbol
ADK
Peptide Modified Sequence
SLIANLAAANC[+57.0]YK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
161
Peptide - Site of Modification
11
Peptide Start
150
Peptide End
162
CPTAC ID
CPTAC-2925
Peptide Molecular Mass
1,407.7180
Species
Homo Sapiens
Assay Type
Direct MRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6.8 2.8 3.7 7.9 3.4 4 10.4 4.4 5.4 15 15 15
y7 (1+) 8.3 3.3 4.4 9.7 3.4 4.4 12.8 4.7 6.2 15 15 15
b3 (1+) 5.3 3.1 4.5 7.2 3.3 4.1 8.9 4.5 6.1 15 15 15
sum 6.3 2.8 4 7.9 3.1 4 10.1 4.2 5.7 15 15 15


Additional Resources and Comments