ADK, CPTAC-162 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ADK	N/A

Sequence Length (AA)	Molecular Weight (Da)
362	40545

Protein Name
Adenosine kinase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAAEEEPKP	KKLKVEAPQA	LRENILFGMG	NPLLDISAVV	DKDFLDKYSL
60	70	80	90	100
KPNDQILAED	KHKELFDELV	KKFKVEYHAG	GSTQNSIKVA	QWMIQQPHKA
110	120	130	140	150
ATFFGCIGID	KFGEILKRKA	AEAHVDAHYY	EQNEQPTGTC	AACITGDNRS
160	170	180	190	200
LIANLAAANC	YKKEKHLDLE	KNWMLVEKAR	VCYIAGFFLT	VSPESVLKVA
210	220	230	240	250
HHASENNRIF	TLNLSAPFIS	QFYKESLMKV	MPYVDILFGN	ETEAATFARE
260	270	280	290	300
QGFETKDIKE	IAKKTQALPK	MNSKRQRIVI	FTQGRDDTIM	ATESEVTAFA
310	320	330	340	350
VLDQDQKEII	DTNGAGDAFV	GGFLSQLVSD	KPLTECIRAG	HYAASIIIRR
360	362
TGCTFPEKPD	FH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-162 Collapse assay details

Data source: Panorama

Official Gene Symbol: ADK
Peptide Modified Sequence: SLIANLAAANC[+57.0]YK
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: 160
Peptide - Site of Modification: 11
Peptide Start: 150
Peptide End: 162

CPTAC ID: CPTAC-162
Peptide Molecular Mass: 1,407.7180
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	13.6	13	12.1	12.4	9.5	15	18.4	16.1	19.3	15	25	15
y7 (1+)	6.1	7	8.4	11.7	10.9	10.8	13.2	13	13.7	15	25	15
y6 (1+)	8.4	7	9	12.3	9.1	12.1	14.9	11.5	15.1	15	25	15
sum	6.3	6.8	8.8	9.4	9	11.2	11.3	11.3	14.2	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2926 Collapse assay details

Data source: Panorama

Official Gene Symbol: ADK
Peptide Sequence: IFTLNLSAPFISQFYK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 209
Peptide End: 224

CPTAC ID: CPTAC-2926
Peptide Molecular Mass: 1,888.0135
Species: Homo Sapiens
Assay Type: Direct MRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend