ADSS, CPTAC-163 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ADSS	ADSS2

Sequence Length (AA)	Molecular Weight (Da)
456	50097

Protein Name
Adenylosuccinate synthetase isozyme 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAFAETYPAA	SSLPNGDCGR	PRARPGGNRV	TVVLGAQWGD	EGKGKVVDLL
60	70	80	90	100
AQDADIVCRC	QGGNNAGHTV	VVDSVEYDFH	LLPSGIINPN	VTAFIGNGVV
110	120	130	140	150
IHLPGLFEEA	EKNVQKGKGL	EGWEKRLIIS	DRAHIVFDFH	QAADGIQEQQ
160	170	180	190	200
RQEQAGKNLG	TTKKGIGPVY	SSKAARSGLR	MCDLVSDFDG	FSERFKVLAN
210	220	230	240	250
QYKSIYPTLE	IDIEGELQKL	KGYMEKIKPM	VRDGVYFLYE	ALHGPPKKIL
260	270	280	290	300
VEGANAALLD	IDFGTYPFVT	SSNCTVGGVC	TGLGMPPQNV	GEVYGVVKAY
310	320	330	340	350
TTRVGIGAFP	TEQDNEIGEL	LQTRGREFGV	TTGRKRRCGW	LDLVLLKYAH
360	370	380	390	400
MINGFTALAL	TKLDILDMFT	EIKVGVAYKL	DGEIIPHIPA	NQEVLNKVEV
410	420	430	440	450
QYKTLPGWNT	DISNARAFKE	LPVNAQNYVR	FIEDELQIPV	KWIGVGKSRE
456
SMIQLF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-164 Collapse assay details

Data source: Panorama

Official Gene Symbol: ADSS
Peptide Sequence: FIEDELQIPVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 431
Peptide End: 441

CPTAC ID: CPTAC-164
Peptide Molecular Mass: 1,329.7180
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	6.6	5.9	5	11.3	8.2	4.6	13.1	10.1	6.8	15	25	15
y8 (1+)	7.3	6.8	8.6	13.2	8	8.2	15.1	10.5	11.9	15	25	15
y6 (1+)	13.7	7.6	9.1	17.1	10.2	9.9	21.9	12.7	13.4	15	25	15
sum	5	5.8	5.1	10.8	7.9	4.6	11.9	9.8	6.9	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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