Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ARPC2 ARC34
Sequence Length (AA) Molecular Weight (Da)
300 34333
Protein Name
Actin-related protein 2/3 complex subunit 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MILLEVNNRI IEETLALKFE NAAAGNKPEA VEVTFADFDG VLYHISNPNG
60 70 80 90 100
DKTKVMVSIS LKFYKELQAH GADELLKRVY GSFLVNPESG YNVSLLYDLE
110 120 130 140 150
NLPASKDSIV HQAGMLKRNC FASVFEKYFQ FQEEGKEGEN RAVIHYRDDE
160 170 180 190 200
TMYVESKKDR VTVVFSTVFK DDDDVVIGKV FMQEFKEGRR ASHTAPQVLF
210 220 230 240 250
SHREPPLELK DTDAAVGDNI GYITFVLFPR HTNASARDNT INLIHTFRDY
260 270 280 290 300
LHYHIKCSKA YIHTRMRAKT SDFLKVLNRA RPDAEKKEMK TITGKTFSSR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-168 Collapse assay details

Data source: Panorama

Official Gene Symbol
ARPC2
Peptide Sequence
DNTINLIHTFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
238
Peptide End
248
CPTAC ID
CPTAC-168
Peptide Molecular Mass
1,342.6993
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 17.2 11.3 17.3 19 13 27 25.6 17.2 32.1 25 15 15
y7 (1+) 9 8.5 8.4 10.3 5.1 10.5 13.7 9.9 13.4 25 15 15
y8 (1+) 14.8 14.3 20 16.8 14 31.4 22.4 20 37.2 25 15 15
sum 7.7 7.4 9.3 7.4 4.7 11.8 10.7 8.8 15 25 15 15

Additional Resources and Comments


Assay Details for CPTAC-167 Collapse assay details

Data source: Panorama

Official Gene Symbol
ARPC2
Peptide Modified Sequence
NC[+57.0]FASVFEK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
120
Peptide - Site of Modification
2
Peptide Start
119
Peptide End
127
CPTAC ID
CPTAC-167
Peptide Molecular Mass
1,100.4961
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 5.4 6.9 8.6 6 10.7 7.7 8.1 12.7 11.5 15 25 15
y6 (1+) 7.5 7 8 7.8 7.2 7.7 10.8 10 11.1 15 25 15
y5 (1+) 8.8 6.7 7.1 13 7 9.3 15.7 9.7 11.7 15 25 15
sum 5.5 6.1 7.6 5.9 8 7.2 8.1 10.1 10.5 15 25 15

Additional Resources and Comments