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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CTSB CPSB
Sequence Length (AA) Molecular Weight (Da)
339 37822
Protein Name
Cathepsin B
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWQLWASLCC LLVLANARSR PSFHPLSDEL VNYVNKRNTT WQAGHNFYNV
60 70 80 90 100
DMSYLKRLCG TFLGGPKPPQ RVMFTEDLKL PASFDAREQW PQCPTIKEIR
110 120 130 140 150
DQGSCGSCWA FGAVEAISDR ICIHTNAHVS VEVSAEDLLT CCGSMCGDGC
160 170 180 190 200
NGGYPAEAWN FWTRKGLVSG GLYESHVGCR PYSIPPCEHH VNGSRPPCTG
210 220 230 240 250
EGDTPKCSKI CEPGYSPTYK QDKHYGYNSY SVSNSEKDIM AEIYKNGPVE
260 270 280 290 300
GAFSVYSDFL LYKSGVYQHV TGEMMGGHAI RILGWGVENG TPYWLVANSW
310 320 330 339
NTDWGDNGFF KILRGQDHCG IESEVVAGIP RTDQYWEKI

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-186 Collapse assay details

Data source: Panorama

Official Gene Symbol
CTSB
Peptide Modified Sequence
IC[+57.0]EPGYSPTYK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
211
Peptide - Site of Modification
2
Peptide Start
210
Peptide End
220
CPTAC ID
CPTAC-186
Peptide Molecular Mass
1,313.5962
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 4.6 7 9.9 10.8 14.3 12.8 11.7 15.9 16.2 15 25 15
y8 (1+) 5.1 6.6 9.2 10.3 12.4 12.4 11.5 14 15.4 15 25 15
y7 (1+) 7.8 6.5 10.2 15.3 8.4 12 17.2 10.6 15.7 15 25 15
sum 4.4 6.4 9.3 10.4 12.4 12.3 11.3 14 15.4 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-187 Collapse assay details

Data source: Panorama

Official Gene Symbol
CTSB
Peptide Sequence
NGPVEGAFSVYSDFLLYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
246
Peptide End
263
CPTAC ID
CPTAC-187
Peptide Molecular Mass
2,004.9833
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 38.3 21 25.8 40.3 26.4 30.2 55.6 33.7 39.7 15 15 15
y8 (1+) 26.4 18.5 14.1 27.1 20.9 17.4 37.8 27.9 22.4 15 15 15
y6 (1+) 37.4 32.1 21.4 55.5 30.2 45.1 66.9 44.1 49.9 15 15 15
sum 28.5 14.8 9.3 31.6 22.9 17.3 42.6 27.3 19.6 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3564 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ctsb
Peptide Sequence
HFGYTSYSVSNSVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
224
Peptide End
237
CPTAC ID
non-CPTAC-3564
Peptide Molecular Mass
1,574.7365
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 8.5 8.4 4.8 9.2 12.1 5 12.5 14.7 6.9 15 15 15
y8 (2+) 18.7 8.6 9.9 29 11.3 9.1 34.5 14.2 13.4 15 15 15
y5 (1+) 14.9 4.4 4.7 14.8 9.5 5.6 21 10.5 7.3 15 15 15
y6 (1+) 8.7 5.1 7.2 17.5 9.2 7.3 19.5 10.5 10.3 15 15 15
y7 (1+) 11.2 5.8 5.8 12.2 5.6 6.5 16.6 8.1 8.7 15 15 15
sum 7.2 2.1 3.1 13.7 6.1 3.5 15.5 6.5 4.7 15 15 15

Additional Resources and Comments