HUWE1, CPTAC-218 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
HUWE1	KIAA0312, KIAA1578, UREB1

Sequence Length (AA)	Molecular Weight (Da)
4374	481891

Protein Name
E3 ubiquitin-protein ligase HUWE1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKVDRTKLKK	TPTEAPADCR	ALIDKLKVCN	DEQLLLELQQ	IKTWNIGKCE
60	70	80	90	100
LYHWVDLLDR	FDGILADAGQ	TVENMSWMLV	CDRPEREQLK	MLLLAVLNFT
110	120	130	140	150
ALLIEYSFSR	HLYSSIEHLT	TLLASSDMQV	VLAVLNLLYV	FSKRSNYITR
160	170	180	190	200
LGSDKRTPLL	TRLQHLAESW	GGKENGFGLA	ECCRDLHMMK	YPPSATTLHF
210	220	230	240	250
EFYADPGAEV	KIEKRTTSNT	LHYIHIEQLD	KISESPSEIM	ESLTKMYSIP
260	270	280	290	300
KDKQMLLFTH	IRLAHGFSNH	RKRLQAVQAR	LHAISILVYS	NALQESANSI
310	320	330	340	350
LYNGLIEELV	DVLQITDKQL	MEIKAASLRT	LTSIVHLERT	PKLSSIIDCT
360	370	380	390	400
GTASYHGFLP	VLVRNCIQAM	IDPSMDPYPH	QFATALFSFL	YHLASYDAGG
410	420	430	440	450
EALVSCGMME	ALLKVIKFLG	DEQDQITFVT	RAVRVVDLIT	NLDMAAFQSH
460	470	480	490	500
SGLSIFIYRL	EHEVDLCRKE	CPFVIKPKIQ	RPNTTQEGEE	METDMDGVQC
510	520	530	540	550
IPQRAALLKS	MLNFLKKAIQ	DPAFSDGIRH	VMDGSLPTSL	KHIISNAEYY
560	570	580	590	600
GPSLFLLATE	VVTVFVFQEP	SLLSSLQDNG	LTDVMLHALL	IKDVPATREV
610	620	630	640	650
LGSLPNVFSA	LCLNARGLQS	FVQCQPFERL	FKVLLSPDYL	PAMRRRRSSD
660	670	680	690	700
PLGDTASNLG	SAVDELMRHQ	PTLKTDATTA	IIKLLEEICN	LGRDPKYICQ
710	720	730	740	750
KPSIQKADGT	ATAPPPRSNH	AAEEASSEDE	EEEEVQAMQS	FNSTQQNETE
760	770	780	790	800
PNQQVVGTEE	RIPIPLMDYI	LNVMKFVESI	LSNNTTDDHC	QEFVNQKGLL
810	820	830	840	850
PLVTILGLPN	LPIDFPTSAA	CQAVAGVCKS	ILTLSHEPKV	LQEGLLQLDS
860	870	880	890	900
ILSSLEPLHR	PIESPGGSVL	LRELACAGNV	ADATLSAQAT	PLLHALTAAH
910	920	930	940	950
AYIMMFVHTC	RVGQSEIRSI	SVNQWGSQLG	LSVLSKLSQL	YCSLVWESTV
960	970	980	990	1000
LLSLCTPNSL	PSGCEFGQAD	MQKLVPKDEK	AGTTQGGKRS	DGEQDGAAGS
1010	1020	1030	1040	1050
MDASTQGLLE	GIGLDGDTLA	PMETDEPTAS	DSKGKSKITP	AMAARIKQIK
1060	1070	1080	1090	1100
PLLSASSRLG	RALAELFGLL	VKLCVGSPVR	QRRSHHAAST	TTAPTPAARS
1110	1120	1130	1140	1150
TASALTKLLT	KGLSWQPPPY	TPTPRFRLTF	FICSVGFTSP	MLFDERKYPY
1160	1170	1180	1190	1200
HLMLQKFLCS	GGHNALFETF	NWALSMGGKV	PVSEGLEHSD	LPDGTGEFLD
1210	1220	1230	1240	1250
AWLMLVEKMV	NPTTVLESPH	SLPAKLPGGV	QNFPQFSALR	FLVVTQKAAF
1260	1270	1280	1290	1300
TCIKNLWNRK	PLKVYGGRMA	ESMLAILCHI	LRGEPVIRER	LSKEKEGSRG
1310	1320	1330	1340	1350
EEDTGQEEGG	SRREPQVNQQ	QLQQLMDMGF	TREHAMEALL	NTSTMEQATE
1360	1370	1380	1390	1400
YLLTHPPPIM	GGVVRDLSMS	EEDQMMRAIA	MSLGQDIPMD	QRAESPEEVA
1410	1420	1430	1440	1450
CRKEEEERKA	REKQEEEEAK	CLEKFQDADP	LEQDELHTFT	DTMLPGCFHL
1460	1470	1480	1490	1500
LDELPDTVYR	VCDLIMTAIK	RNGADYRDMI	LKQVVNQVWE	AADVLIKAAL
1510	1520	1530	1540	1550
PLTTSDTKTV	SEWISQMATL	PQASNLATRI	LLLTLLFEEL	KLPCAWVVES
1560	1570	1580	1590	1600
SGILNVLIKL	LEVVQPCLQA	AKEQKEVQTP	KWITPVLLLI	DFYEKTAISS
1610	1620	1630	1640	1650
KRRAQMTKYL	QSNSNNWRWF	DDRSGRWCSY	SASNNSTIDS	AWKSGETSVR
1660	1670	1680	1690	1700
FTAGRRRYTV	QFTTMVQVNE	ETGNRRPVML	TLLRVPRLNK	NSKNSNGQEL
1710	1720	1730	1740	1750
EKTLEESKEM	DIKRKENKGN	DTPLALESTN	TEKETSLEET	KIGEILIQGL
1760	1770	1780	1790	1800
TEDMVTVLIR	ACVSMLGVPV	DPDTLHATLR	LCLRLTRDHK	YAMMFAELKS
1810	1820	1830	1840	1850
TRMILNLTQS	SGFNGFTPLV	TLLLRHIIED	PCTLRHTMEK	VVRSAATSGA
1860	1870	1880	1890	1900
GSTTSGVVSG	SLGSREINYI	LRVLGPAACR	NPDIFTEVAN	CCIRIALPAP
1910	1920	1930	1940	1950
RGSGTASDDE	FENLRIKGPN	AVQLVKTTPL	KPSPLPVIPD	TIKEVIYDML
1960	1970	1980	1990	2000
NALAAYHAPE	EADKSDPKPG	VMTQEVGQLL	QDMGDDVYQQ	YRSLTRQSSD
2010	2020	2030	2040	2050
FDTQSGFSIN	SQVFAADGAS	TETSASGTSQ	GEASTPEESR	DGKKDKEGDR
2060	2070	2080	2090	2100
ASEEGKQKGK	GSKPLMPTST	ILRLLAELVR	SYVGIATLIA	NYSYTVGQSE
2110	2120	2130	2140	2150
LIKEDCSVLA	FVLDHLLPHT	QNAEDKDTPA	LARLFLASLA	AAGSGTDAQV
2160	2170	2180	2190	2200
ALVNEVKAAL	GRALAMAEST	EKHARLQAVM	CIISTIMESC	PSTSSFYSSA
2210	2220	2230	2240	2250
TAKTQHNGMN	NIIRLFLKKG	LVNDLARVPH	SLDLSSPNMA	NTVNAALKPL
2260	2270	2280	2290	2300
ETLSRIVNQP	SSLFGSKSAS	SKNKSEQDAQ	GASQDSSSNQ	QDPGEPGEAE
2310	2320	2330	2340	2350
VQEEDHDVTQ	TEVADGDIMD	GEAETDSVVI	AGQPEVLSSQ	EMQVENELED
2360	2370	2380	2390	2400
LIDELLERDG	GSGNSTIIVS	RSGEDESQED	VLMDEAPSNL	SQASTLQANR
2410	2420	2430	2440	2450
EDSMNILDPE	DEEEHTQEED	SSGSNEDEDD	SQDEEEEEEE	DEEDDQEDDE
2460	2470	2480	2490	2500
GEEGDEDDDD	DGSEMELDED	YPDMNASPLV	RFERFDREDD	LIIEFDNMFS
2510	2520	2530	2540	2550
SATDIPPSPG	NIPTTHPLMV	RHADHSSLTL	GSGSSTTRLT	QGIGRSQRTL
2560	2570	2580	2590	2600
RQLTANTGHT	IHVHYPGNRQ	PNPPLILQRL	LGPSAAADIL	QLSSSLPLQS
2610	2620	2630	2640	2650
RGRARLLVGN	DDVHIIARSD	DELLDDFFHD	QSTATSQAGT	LSSIPTALTR
2660	2670	2680	2690	2700
WTEECKVLDA	ESMHDCVSVV	KVSIVNHLEF	LRDEELEERR	EKRRKQLAEE
2710	2720	2730	2740	2750
ETKITDKGKE	DKENRDQSAQ	CTASKSNDST	EQNLSDGTPM	PDSYPTTPSS
2760	2770	2780	2790	2800
TDAATSESKE	TLGTLQSSQQ	QPTLPTPPAL	GEVPQELQSP	AGEGGSSTQL
2810	2820	2830	2840	2850
LMPVEPEELG	PTRPSGEAET	TQMELSPAPT	ITSLSPERAE	DSDALTAVSS
2860	2870	2880	2890	2900
QLEGSPMDTS	SLASCTLEEA	VGDTSAAGSS	EQPRAGSSTP	GDAPPAVAEV
2910	2920	2930	2940	2950
QGRSDGSGES	AQPPEDSSPP	ASSESSSTRD	SAVAISGADS	RGILEEPLPS
2960	2970	2980	2990	3000
TSSEEEDPLA	GISLPEGVDP	SFLAALPDDI	RREVLQNQLG	IRPPTRTAPS
3010	3020	3030	3040	3050
TNSSAPAVVG	NPGVTEVSPE	FLAALPPAIQ	EEVLAQQRAE	QQRRELAQNA
3060	3070	3080	3090	3100
SSDTPMDPVT	FIQTLPSDLR	RSVLEDMEDS	VLAVMPPDIA	AEAQALRREQ
3110	3120	3130	3140	3150
EARQRQLMHE	RLFGHSSTSA	LSAILRSPAF	TSRLSGNRGV	QYTRLAVQRG
3160	3170	3180	3190	3200
GTFQMGGSSS	HNRPSGSNVD	TLLRLRGRLL	LDHEALSCLL	VLLFVDEPKL
3210	3220	3230	3240	3250
NTSRLHRVLR	NLCYHAQTRH	WVIRSLLSIL	QRSSESELCI	ETPKLTTSEE
3260	3270	3280	3290	3300
KGKKSSKSCG	SSSHENRPLD	LLHKMESKSS	NQLSWLSVSM	DAALGCRTNI
3310	3320	3330	3340	3350
FQIQRSGGRK	HTEKHASGGS	TVHIHPQAAP	VVCRHVLDTL	IQLAKVFPSH
3360	3370	3380	3390	3400
FTQQRTKETN	CESDRERGNK	ACSPCSSQSS	SSGICTDFWD	LLVKLDNMNV
3410	3420	3430	3440	3450
SRKGKNSVKS	VPVSAGGEGE	TSPYSLEASP	LGQLMNMLSH	PVIRRSSLLT
3460	3470	3480	3490	3500
EKLLRLLSLI	SIALPENKVS	EAQANSGSGA	SSTTTATSTT	STTTTTAAST
3510	3520	3530	3540	3550
TPTPPTAPTP	VTSAPALVAA	TAISTIVVAA	STTVTTPTTA	TTTVSISPTT
3560	3570	3580	3590	3600
KGSKSPAKVS	DGGSSSTDFK	MVSSGLTENQ	LQLSVEVLTS	HSCSEEGLED
3610	3620	3630	3640	3650
AANVLLQLSR	GDSGTRDTVL	KLLLNGARHL	GYTLCKQIGT	LLAELREYNL
3660	3670	3680	3690	3700
EQQRRAQCET	LSPDGLPEEQ	PQTTKLKGKM	QSRFDMAENV	VIVASQKRPL
3710	3720	3730	3740	3750
GGRELQLPSM	SMLTSKTSTQ	KFFLRVLQVI	IQLRDDTRRA	NKKAKQTGRL
3760	3770	3780	3790	3800
GSSGLGSASS	IQAAVRQLEA	EADAIIQMVR	EGQRARRQQQ	AATSESSQSE
3810	3820	3830	3840	3850
ASVRREESPM	DVDQPSPSAQ	DTQSIASDGT	PQGEKEKEER	PPELPLLSEQ
3860	3870	3880	3890	3900
LSLDELWDML	GECLKELEES	HDQHAVLVLQ	PAVEAFFLVH	ATERESKPPV
3910	3920	3930	3940	3950
RDTRESQLAH	IKDEPPPLSP	APLTPATPSS	LDPFFSREPS	SMHISSSLPP
3960	3970	3980	3990	4000
DTQKFLRFAE	THRTVLNQIL	RQSTTHLADG	PFAVLVDYIR	VLDFDVKRKY
4010	4020	4030	4040	4050
FRQELERLDE	GLRKEDMAVH	VRRDHVFEDS	YRELHRKSPE	EMKNRLYIVF
4060	4070	4080	4090	4100
EGEEGQDAGG	LLREWYMIIS	REMFNPMYAL	FRTSPGDRVT	YTINPSSHCN
4110	4120	4130	4140	4150
PNHLSYFKFV	GRIVAKAVYD	NRLLECYFTR	SFYKHILGKS	VRYTDMESED
4160	4170	4180	4190	4200
YHFYQGLVYL	LENDVSTLGY	DLTFSTEVQE	FGVCEVRDLK	PNGANILVTE
4210	4220	4230	4240	4250
ENKKEYVHLV	CQMRMTGAIR	KQLAAFLEGF	YEIIPKRLIS	IFTEQELELL
4260	4270	4280	4290	4300
ISGLPTIDID	DLKSNTEYHK	YQSNSIQIQW	FWRALRSFDQ	ADRAKFLQFV
4310	4320	4330	4340	4350
TGTSKVPLQG	FAALEGMNGI	QKFQIHRDDR	STDRLPSAHT	CFNQLDLPAY
4360	4370	4374
ESFEKLRHML	LLAIQECSEG	FGLA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-218 Collapse assay details

Data source: Panorama

Official Gene Symbol: HUWE1
Peptide Sequence: AGSSTPGDAPPAVAEVQGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 2885
Peptide End: 2903

CPTAC ID: CPTAC-218
Peptide Molecular Mass: 1,765.8595
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	10	9.8	13.8	17.7	10.9	12.3	20.3	14.7	18.5	15	25	15
y10 (1+)	4.5	5.5	4.8	4.6	5.9	5.4	6.4	8.1	7.2	15	25	15
y9 (1+)	14.4	8.9	7.1	15.2	10.6	6.8	20.9	13.8	9.8	15	25	15
sum	4.5	5.6	6	7	6.5	5.9	8.3	8.6	8.4	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments