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Overview Data source: UniProt

Official Gene Symbol Other Aliases
ICAM1 N/A
Sequence Length (AA) Molecular Weight (Da)
532 57825
Protein Name
Intercellular adhesion molecule 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPSSPRPAL PALLVLLGAL FPGPGNAQTS VSPSKVILPR GGSVLVTCST
60 70 80 90 100
SCDQPKLLGI ETPLPKKELL LPGNNRKVYE LSNVQEDSQP MCYSNCPDGQ
110 120 130 140 150
STAKTFLTVY WTPERVELAP LPSWQPVGKN LTLRCQVEGG APRANLTVVL
160 170 180 190 200
LRGEKELKRE PAVGEPAEVT TTVLVRRDHH GANFSCRTEL DLRPQGLELF
210 220 230 240 250
ENTSAPYQLQ TFVLPATPPQ LVSPRVLEVD TQGTVVCSLD GLFPVSEAQV
260 270 280 290 300
HLALGDQRLN PTVTYGNDSF SAKASVSVTA EDEGTQRLTC AVILGNQSQE
310 320 330 340 350
TLQTVTIYSF PAPNVILTKP EVSEGTEVTV KCEAHPRAKV TLNGVPAQPL
360 370 380 390 400
GPRAQLLLKA TPEDNGRSFS CSATLEVAGQ LIHKNQTREL RVLYGPRLDE
410 420 430 440 450
RDCPGNWTWP ENSQQTPMCQ AWGNPLPELK CLKDGTFPLP IGESVTVTRD
460 470 480 490 500
LEGTYLCRAR STQGEVTRKV TVNVLSPRYE IVIITVVAAA VIMGTAGLST
510 520 530 532
YLYNRQRKIK KYRLQQAQKG TPMKPNTQAT PP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-219 Collapse assay details

Data source: Panorama

Official Gene Symbol
ICAM1
Peptide Sequence
VELAPLPSWQPVGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
116
Peptide End
129
CPTAC ID
CPTAC-219
Peptide Molecular Mass
1,519.8399
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 12.2 8.9 9.4 17.4 13.9 16.8 21.3 16.5 19.3 15 15 15
y10 (1+) 7.9 6.6 5.2 15.4 8.3 9 17.3 10.6 10.4 15 15 15
y8 (1+) 6 4.6 6.5 16.2 7.8 10.9 17.3 9.1 12.7 15 15 15
sum 6.9 4.7 4.1 15.6 7.8 9.8 17.1 9.1 10.6 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-220 Collapse assay details

Data source: Panorama

Official Gene Symbol
ICAM1
Peptide Sequence
VTLNGVPAQPLGPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
340
Peptide End
353
CPTAC ID
CPTAC-220
Peptide Molecular Mass
1,417.8041
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 5.7 3.2 4.5 9.3 3.5 5.8 10.9 4.7 7.3 15 15 15
y10 (1+) 5 5.4 6.7 7.4 8 10.3 8.9 9.7 12.3 15 15 15
y8 (1+) 3.8 2.6 3.5 6.1 5.5 11.2 7.2 6.1 11.7 15 15 15
sum 3.7 2.4 3.4 6.1 5.3 10.6 7.1 5.8 11.1 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2679 Collapse assay details

Data source: Panorama

Official Gene Symbol
ICAM1
Peptide Sequence
LLGIETPLPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
57
Peptide End
66
CPTAC ID
non-CPTAC-2679
Peptide Molecular Mass
1,079.6590
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 21.5 9.1 6.2 25.3 10.6 9.2 33.2 14 11.1 15 15 15
y8 (1+) 9.6 6.8 9.3 14 5.5 8.6 17 8.7 12.7 15 15 15
y6 (1+) 12.3 7.7 7 16.6 9.1 9.3 20.7 11.9 11.6 15 15 15
b3 (1+) 15.9 8.4 7 15 10.6 9.5 21.9 13.5 11.8 15 15 15
y4 (1+) 16.7 8.2 8.6 24.6 9.7 9.9 29.7 12.7 13.1 15 15 15
sum 6.1 4.3 7.2 11.8 5.6 7.9 13.3 7.1 10.7 15 15 15

Additional Resources and Comments