MYH14, CPTAC-240 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MYH14	KIAA2034

Sequence Length (AA)	Molecular Weight (Da)
1995	227871

Protein Name
Myosin-14

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAVTMSVPG	RKAPPRPGPV	PEAAQPFLFT	PRGPSAGGGP	GSGTSPQVEW
60	70	80	90	100
TARRLVWVPS	ELHGFEAAAL	RDEGEEEAEV	ELAESGRRLR	LPRDQIQRMN
110	120	130	140	150
PPKFSKAEDM	AELTCLNEAS	VLHNLRERYY	SGLIYTYSGL	FCVVINPYKQ
160	170	180	190	200
LPIYTEAIVE	MYRGKKRHEV	PPHVYAVTEG	AYRSMLQDRE	DQSILCTGES
210	220	230	240	250
GAGKTENTKK	VIQYLAHVAS	SPKGRKEPGV	PGELERQLLQ	ANPILEAFGN
260	270	280	290	300
AKTVKNDNSS	RFGKFIRINF	DVAGYIVGAN	IETYLLEKSR	AIRQAKDECS
310	320	330	340	350
FHIFYQLLGG	AGEQLKADLL	LEPCSHYRFL	TNGPSSSPGQ	ERELFQETLE
360	370	380	390	400
SLRVLGFSHE	EIISMLRMVS	AVLQFGNIAL	KRERNTDQAT	MPDNTAAQKL
410	420	430	440	450
CRLLGLGVTD	FSRALLTPRI	KVGRDYVQKA	QTKEQADFAL	EALAKATYER
460	470	480	490	500
LFRWLVLRLN	RALDRSPRQG	ASFLGILDIA	GFEIFQLNSF	EQLCINYTNE
510	520	530	540	550
KLQQLFNHTM	FVLEQEEYQR	EGIPWTFLDF	GLDLQPCIDL	IERPANPPGL
560	570	580	590	600
LALLDEECWF	PKATDKSFVE	KVAQEQGGHP	KFQRPRHLRD	QADFSVLHYA
610	620	630	640	650
GKVDYKANEW	LMKNMDPLND	NVAALLHQST	DRLTAEIWKD	VEGIVGLEQV
660	670	680	690	700
SSLGDGPPGG	RPRRGMFRTV	GQLYKESLSR	LMATLSNTNP	SFVRCIVPNH
710	720	730	740	750
EKRAGKLEPR	LVLDQLRCNG	VLEGIRICRQ	GFPNRILFQE	FRQRYEILTP
760	770	780	790	800
NAIPKGFMDG	KQACEKMIQA	LELDPNLYRV	GQSKIFFRAG	VLAQLEEERD
810	820	830	840	850
LKVTDIIVSF	QAAARGYLAR	RAFQKRQQQQ	SALRVMQRNC	AAYLKLRHWQ
860	870	880	890	900
WWRLFTKVKP	LLQVTRQDEV	LQARAQELQK	VQELQQQSAR	EVGELQGRVA
910	920	930	940	950
QLEEERARLA	EQLRAEAELC	AEAEETRGRL	AARKQELELV	VSELEARVGE
960	970	980	990	1000
EEECSRQMQT	EKKRLQQHIQ	ELEAHLEAEE	GARQKLQLEK	VTTEAKMKKF
1010	1020	1030	1040	1050
EEDLLLLEDQ	NSKLSKERKL	LEDRLAEFSS	QAAEEEEKVK	SLNKLRLKYE
1060	1070	1080	1090	1100
ATIADMEDRL	RKEEKGRQEL	EKLKRRLDGE	SSELQEQMVE	QQQRAEELRA
1110	1120	1130	1140	1150
QLGRKEEELQ	AALARAEDEG	GARAQLLKSL	REAQAALAEA	QEDLESERVA
1160	1170	1180	1190	1200
RTKAEKQRRD	LGEELEALRG	ELEDTLDSTN	AQQELRSKRE	QEVTELKKTL
1210	1220	1230	1240	1250
EEETRIHEAA	VQELRQRHGQ	ALGELAEQLE	QARRGKGAWE	KTRLALEAEV
1260	1270	1280	1290	1300
SELRAELSSL	QTARQEGEQR	RRRLELQLQE	VQGRAGDGER	ARAEAAEKLQ
1310	1320	1330	1340	1350
RAQAELENVS	GALNEAESKT	IRLSKELSST	EAQLHDAQEL	LQEETRAKLA
1360	1370	1380	1390	1400
LGSRVRAMEA	EAAGLREQLE	EEAAARERAG	RELQTAQAQL	SEWRRRQEEE
1410	1420	1430	1440	1450
AGALEAGEEA	RRRAAREAEA	LTQRLAEKTE	TVDRLERGRR	RLQQELDDAT
1460	1470	1480	1490	1500
MDLEQQRQLV	STLEKKQRKF	DQLLAEEKAA	VLRAVEERER	AEAEGREREA
1510	1520	1530	1540	1550
RALSLTRALE	EEQEAREELE	RQNRALRAEL	EALLSSKDDV	GKSVHELERA
1560	1570	1580	1590	1600
CRVAEQAAND	LRAQVTELED	ELTAAEDAKL	RLEVTVQALK	TQHERDLQGR
1610	1620	1630	1640	1650
DEAGEERRRQ	LAKQLRDAEV	ERDEERKQRT	LAVAARKKLE	GELEELKAQM
1660	1670	1680	1690	1700
ASAGQGKEEA	VKQLRKMQAQ	MKELWREVEE	TRTSREEIFS	QNRESEKRLK
1710	1720	1730	1740	1750
GLEAEVLRLQ	EELAASDRAR	RQAQQDRDEM	ADEVANGNLS	KAAILEEKRQ
1760	1770	1780	1790	1800
LEGRLGQLEE	ELEEEQSNSE	LLNDRYRKLL	LQVESLTTEL	SAERSFSAKA
1810	1820	1830	1840	1850
ESGRQQLERQ	IQELRGRLGE	EDAGARARHK	MTIAALESKL	AQAEEQLEQE
1860	1870	1880	1890	1900
TRERILSGKL	VRRAEKRLKE	VVLQVEEERR	VADQLRDQLE	KGNLRVKQLK
1910	1920	1930	1940	1950
RQLEEAEEEA	SRAQAGRRRL	QRELEDVTES	AESMNREVTT	LRNRLRRGPL
1960	1970	1980	1990	1995
TFTTRTVRQV	FRLEEGVASD	EEAEEAQPGS	GPSPEPEGSP	PAHPQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-240 Collapse assay details

Data source: Panorama

Official Gene Symbol: MYH14
Peptide Sequence: AQAELENVSGALNEAESK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 1302
Peptide End: 1319

CPTAC ID: CPTAC-240
Peptide Molecular Mass: 1,858.8909
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (1+)	10.2	13.4	11.3	15.6	17.8	21.1	18.6	22.3	23.9	15	25	15
y11 (1+)	14.5	13.2	12.6	16	17.4	13.2	21.6	21.8	18.2	15	25	15
y10 (1+)	7.2	9.8	8.1	9.9	13.8	10.1	12.2	16.9	12.9	15	25	15
sum	6	7.6	6.4	8.2	13.2	9.3	10.2	15.2	11.3	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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