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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MYH14 KIAA2034
Sequence Length (AA) Molecular Weight (Da)
1995 227871
Protein Name
Myosin-14
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAVTMSVPG RKAPPRPGPV PEAAQPFLFT PRGPSAGGGP GSGTSPQVEW
60 70 80 90 100
TARRLVWVPS ELHGFEAAAL RDEGEEEAEV ELAESGRRLR LPRDQIQRMN
110 120 130 140 150
PPKFSKAEDM AELTCLNEAS VLHNLRERYY SGLIYTYSGL FCVVINPYKQ
160 170 180 190 200
LPIYTEAIVE MYRGKKRHEV PPHVYAVTEG AYRSMLQDRE DQSILCTGES
210 220 230 240 250
GAGKTENTKK VIQYLAHVAS SPKGRKEPGV PGELERQLLQ ANPILEAFGN
260 270 280 290 300
AKTVKNDNSS RFGKFIRINF DVAGYIVGAN IETYLLEKSR AIRQAKDECS
310 320 330 340 350
FHIFYQLLGG AGEQLKADLL LEPCSHYRFL TNGPSSSPGQ ERELFQETLE
360 370 380 390 400
SLRVLGFSHE EIISMLRMVS AVLQFGNIAL KRERNTDQAT MPDNTAAQKL
410 420 430 440 450
CRLLGLGVTD FSRALLTPRI KVGRDYVQKA QTKEQADFAL EALAKATYER
460 470 480 490 500
LFRWLVLRLN RALDRSPRQG ASFLGILDIA GFEIFQLNSF EQLCINYTNE
510 520 530 540 550
KLQQLFNHTM FVLEQEEYQR EGIPWTFLDF GLDLQPCIDL IERPANPPGL
560 570 580 590 600
LALLDEECWF PKATDKSFVE KVAQEQGGHP KFQRPRHLRD QADFSVLHYA
610 620 630 640 650
GKVDYKANEW LMKNMDPLND NVAALLHQST DRLTAEIWKD VEGIVGLEQV
660 670 680 690 700
SSLGDGPPGG RPRRGMFRTV GQLYKESLSR LMATLSNTNP SFVRCIVPNH
710 720 730 740 750
EKRAGKLEPR LVLDQLRCNG VLEGIRICRQ GFPNRILFQE FRQRYEILTP
760 770 780 790 800
NAIPKGFMDG KQACEKMIQA LELDPNLYRV GQSKIFFRAG VLAQLEEERD
810 820 830 840 850
LKVTDIIVSF QAAARGYLAR RAFQKRQQQQ SALRVMQRNC AAYLKLRHWQ
860 870 880 890 900
WWRLFTKVKP LLQVTRQDEV LQARAQELQK VQELQQQSAR EVGELQGRVA
910 920 930 940 950
QLEEERARLA EQLRAEAELC AEAEETRGRL AARKQELELV VSELEARVGE
960 970 980 990 1000
EEECSRQMQT EKKRLQQHIQ ELEAHLEAEE GARQKLQLEK VTTEAKMKKF
1010 1020 1030 1040 1050
EEDLLLLEDQ NSKLSKERKL LEDRLAEFSS QAAEEEEKVK SLNKLRLKYE
1060 1070 1080 1090 1100
ATIADMEDRL RKEEKGRQEL EKLKRRLDGE SSELQEQMVE QQQRAEELRA
1110 1120 1130 1140 1150
QLGRKEEELQ AALARAEDEG GARAQLLKSL REAQAALAEA QEDLESERVA
1160 1170 1180 1190 1200
RTKAEKQRRD LGEELEALRG ELEDTLDSTN AQQELRSKRE QEVTELKKTL
1210 1220 1230 1240 1250
EEETRIHEAA VQELRQRHGQ ALGELAEQLE QARRGKGAWE KTRLALEAEV
1260 1270 1280 1290 1300
SELRAELSSL QTARQEGEQR RRRLELQLQE VQGRAGDGER ARAEAAEKLQ
1310 1320 1330 1340 1350
RAQAELENVS GALNEAESKT IRLSKELSST EAQLHDAQEL LQEETRAKLA
1360 1370 1380 1390 1400
LGSRVRAMEA EAAGLREQLE EEAAARERAG RELQTAQAQL SEWRRRQEEE
1410 1420 1430 1440 1450
AGALEAGEEA RRRAAREAEA LTQRLAEKTE TVDRLERGRR RLQQELDDAT
1460 1470 1480 1490 1500
MDLEQQRQLV STLEKKQRKF DQLLAEEKAA VLRAVEERER AEAEGREREA
1510 1520 1530 1540 1550
RALSLTRALE EEQEAREELE RQNRALRAEL EALLSSKDDV GKSVHELERA
1560 1570 1580 1590 1600
CRVAEQAAND LRAQVTELED ELTAAEDAKL RLEVTVQALK TQHERDLQGR
1610 1620 1630 1640 1650
DEAGEERRRQ LAKQLRDAEV ERDEERKQRT LAVAARKKLE GELEELKAQM
1660 1670 1680 1690 1700
ASAGQGKEEA VKQLRKMQAQ MKELWREVEE TRTSREEIFS QNRESEKRLK
1710 1720 1730 1740 1750
GLEAEVLRLQ EELAASDRAR RQAQQDRDEM ADEVANGNLS KAAILEEKRQ
1760 1770 1780 1790 1800
LEGRLGQLEE ELEEEQSNSE LLNDRYRKLL LQVESLTTEL SAERSFSAKA
1810 1820 1830 1840 1850
ESGRQQLERQ IQELRGRLGE EDAGARARHK MTIAALESKL AQAEEQLEQE
1860 1870 1880 1890 1900
TRERILSGKL VRRAEKRLKE VVLQVEEERR VADQLRDQLE KGNLRVKQLK
1910 1920 1930 1940 1950
RQLEEAEEEA SRAQAGRRRL QRELEDVTES AESMNREVTT LRNRLRRGPL
1960 1970 1980 1990 1995
TFTTRTVRQV FRLEEGVASD EEAEEAQPGS GPSPEPEGSP PAHPQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-240 Collapse assay details

Data source: Panorama

Official Gene Symbol
MYH14
Peptide Sequence
AQAELENVSGALNEAESK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1302
Peptide End
1319
CPTAC ID
CPTAC-240
Peptide Molecular Mass
1,858.8909
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 10.2 13.4 11.3 15.6 17.8 21.1 18.6 22.3 23.9 15 25 15
y11 (1+) 14.5 13.2 12.6 16 17.4 13.2 21.6 21.8 18.2 15 25 15
y10 (1+) 7.2 9.8 8.1 9.9 13.8 10.1 12.2 16.9 12.9 15 25 15
sum 6 7.6 6.4 8.2 13.2 9.3 10.2 15.2 11.3 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-241 Collapse assay details

Data source: Panorama

Official Gene Symbol
MYH14
Peptide Sequence
ELSSTEAQLHDAQELLQEETR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1326
Peptide End
1346
CPTAC ID
CPTAC-241
Peptide Molecular Mass
2,426.1561
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y19 (2+) 26.9 12 11.7 24 16.1 20.1 36.1 20.1 23.3 15 25 15
y16 (2+) 43.6 16.2 21.3 44.9 19.1 22.8 62.6 25 31.2 15 25 15
y15 (2+) 42.3 21.8 14.4 46.1 25 16 62.6 33.2 21.5 15 25 15
sum 20 9.3 11.8 22 14.4 16.1 29.7 17.1 20 15 25 15

Additional Resources and Comments