NAGK, CPTAC-242 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NAGK	N/A

Sequence Length (AA)	Molecular Weight (Da)
344	37376

Protein Name
N-acetyl-D-glucosamine kinase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAIYGGVEG	GGTRSEVLLV	SEDGKILAEA	DGLSTNHWLI	GTDKCVERIN
60	70	80	90	100
EMVNRAKRKA	GVDPLVPLRS	LGLSLSGGDQ	EDAGRILIEE	LRDRFPYLSE
110	120	130	140	150
SYLITTDAAG	SIATATPDGG	VVLISGTGSN	CRLINPDGSE	SGCGGWGHMM
160	170	180	190	200
GDEGSAYWIA	HQAVKIVFDS	IDNLEAAPHD	IGYVKQAMFH	YFQVPDRLGI
210	220	230	240	250
LTHLYRDFDK	CRFAGFCRKI	AEGAQQGDPL	SRYIFRKAGE	MLGRHIVAVL
260	270	280	290	300
PEIDPVLFQG	KIGLPILCVG	SVWKSWELLK	EGFLLALTQG	REIQAQNFFS
310	320	330	340	344
SFTLMKLRHS	SALGGASLGA	RHIGHLLPMD	YSANAIAFYS	YTFS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-243 Collapse assay details

Data source: Panorama

Official Gene Symbol: NAGK
Peptide Sequence: IAEGAQQGDPLSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 220
Peptide End: 232

CPTAC ID: CPTAC-243
Peptide Molecular Mass: 1,340.6684
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	3.5	5.7	6	8.1	12.3	8.9	8.8	13.6	10.7	15	25	15
y8 (1+)	5.3	5.7	6.9	10.1	11.5	11.5	11.4	12.8	13.4	15	25	15
y7 (1+)	7	5.7	5.4	10.8	13	9.5	12.9	14.2	10.9	15	25	15
sum	4.7	4.9	5	9.6	12.1	9.6	10.7	13.1	10.8	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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