Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
AEN ISG20L1
Sequence Length (AA) Molecular Weight (Da)
325 36350
Protein Name
Apoptosis-enhancing nuclease
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVPREAPESA QCLCPSLTIP NAKDVLRKRH KRRSRQHQRF MARKALLQEQ
60 70 80 90 100
GLLSMPPEPG SSPLPTPFGA ATATEAASSG KQCLRAGSGS APCSRRPAPG
110 120 130 140 150
KASGPLPSKC VAIDCEMVGT GPRGRVSELA RCSIVSYHGN VLYDKYIRPE
160 170 180 190 200
MPIADYRTRW SGITRQHMRK AVPFQVAQKE ILKLLKGKVV VGHALHNDFQ
210 220 230 240 250
ALKYVHPRSQ TRDTTYVPNF LSEPGLHTRA RVSLKDLALQ LLHKKIQVGQ
260 270 280 290 300
HGHSSVEDAT TAMELYRLVE VQWEQQEARS LWTCPEDREP DSSTDMEQYM
310 320 325
EDQYWPDDLA HGSRGGAREA QDRRN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-2613 Collapse assay details

Data source: Panorama

Official Gene Symbol
AEN
Peptide Modified Sequence
LN[+1.0]STAQK
Modification Type
Deamidated (N)
Protein - Site of Modification
2
Peptide - Site of Modification
2
Peptide Start
1
Peptide End
7
CPTAC ID
CPTAC-2613
Peptide Molecular Mass
761.3919
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-H


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 34.7 9.3 5.1 40.5 7.4 6.7 53.3 11.9 8.4 15 15 15
y5 (1+) 8.2 1.9 2.7 7.2 1.6 3 10.9 2.5 4 15 15 15
y6 (1+) 8.8 3.1 3.1 8.7 3.9 3.5 12.4 5 4.7 15 15 15
sum 7.5 1.3 2.4 6.8 1.6 2.7 10.1 2.1 3.6 15 15 15


Additional Resources and Comments