GNPTG, CPTAC-2710 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GNPTG	C16orf27, GNPTAG

Sequence Length (AA)	Molecular Weight (Da)
305	33974

Protein Name
N-acetylglucosamine-1-phosphotransferase subunit gamma

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAGLARLLL	LLGLSAGGPA	PAGAAKMKVV	EEPNAFGVNN	PFLPQASRLQ
60	70	80	90	100
AKRDPSPVSG	PVHLFRLSGK	CFSLVESTYK	YEFCPFHNVT	QHEQTFRWNA
110	120	130	140	150
YSGILGIWHE	WEIANNTFTG	MWMRDGDACR	SRSRQSKVEL	ACGKSNRLAH
160	170	180	190	200
VSEPSTCVYA	LTFETPLVCH	PHALLVYPTL	PEALQRQWDQ	VEQDLADELI
210	220	230	240	250
TPQGHEKLLR	TLFEDAGYLK	TPEENEPTQL	EGGPDSLGFE	TLENCRKAHK
260	270	280	290	300
ELSKEIKRLK	GLLTQHGIPY	TRPTETSNLE	HLGHETPRAK	SPEQLRGDPG
305
LRGSL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2710 Collapse assay details

Data source: Panorama

Official Gene Symbol: GNPTG
Peptide Modified Sequence: YEFC[+57.0]PFHN[+1.0]VTQHEQTFR
Modification Type: Carbamidomethyl (C), Deamidated (N)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4, 8
Peptide Start: 81
Peptide End: 97

CPTAC ID: CPTAC-2710
Peptide Molecular Mass: 2,239.9745
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-H

CPTAC-2613:

AEN.

LN[+1.0]STAQK

CPTAC-2710:

GNPTG.

YEFC[+57.0]PFHN[+1.0]VTQHEQTFR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y13 (2+)	9.6	10.5	6.3	8.7	11.5	9.8	13	15.6	11.7	15	15	15
y11 (2+)	8.6	7.7	10.4	10.1	8.4	10.5	13.3	11.4	14.8	15	15	15
y6 (2+)	12.4	12.7	18.6	14.1	13.5	18.6	18.8	18.5	26.3	15	15	15
sum	4.1	6.5	5.1	5	6.7	4.9	6.5	9.3	7.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3984 Collapse assay details

Data source: Panorama

Official Gene Symbol: Gnptg
Peptide Sequence: VPGETHPTQLAGGSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 221
Peptide End: 235

CPTAC ID: non-CPTAC-3984
Peptide Molecular Mass: 1,477.7525
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers