Gnptg, CPTAC-3984 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Gnptg	Mdcp1

Sequence Length (AA)	Molecular Weight (Da)
307	34169

Protein Name
N-acetylglucosamine-1-phosphotransferase subunit gamma

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGRLAGFLM	LLGLASQGPA	PAYAGKMKVV	EEPNTFGLNN	PFLPQASRLQ
60	70	80	90	100
PKREPSAVSG	PLHLFRLAGK	CFSLVESTYK	YEFCPFHNVT	QHEQTFRWNA
110	120	130	140	150
YSGILGIWHE	WEIINNTFKG	MWMTDGDSCH	SRSRQSKVEL	TCGKINRLAH
160	170	180	190	200
VSEPSTCVYA	LTFETPLVCH	PHSLLVYPTL	SEALQQRWDQ	VEQDLADELI
210	220	230	240	250
TPQGYEKLLR	VLFEDAGYLK	VPGETHPTQL	AGGSKGLGLE	TLDNCRKAHA
260	270	280	290	300
ELSQEVQRLT	SLLQQHGIPH	TQPTETTHSQ	HLGQQLPIGA	IAAEHLRSDP
307
GLRGNIL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2710 Collapse assay details

Data source: Panorama

Official Gene Symbol: GNPTG
Peptide Modified Sequence: YEFC[+57.0]PFHN[+1.0]VTQHEQTFR
Modification Type: Carbamidomethyl (C), Deamidated (N)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4, 8
Peptide Start: 81
Peptide End: 97

CPTAC ID: CPTAC-2710
Peptide Molecular Mass: 2,239.9745
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y13 (2+)	9.6	10.5	6.3	8.7	11.5	9.8	13	15.6	11.7	15	15	15
y11 (2+)	8.6	7.7	10.4	10.1	8.4	10.5	13.3	11.4	14.8	15	15	15
y6 (2+)	12.4	12.7	18.6	14.1	13.5	18.6	18.8	18.5	26.3	15	15	15
sum	4.1	6.5	5.1	5	6.7	4.9	6.5	9.3	7.1	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3984 Collapse assay details

Data source: Panorama

Official Gene Symbol: Gnptg
Peptide Sequence: VPGETHPTQLAGGSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 221
Peptide End: 235

CPTAC ID: non-CPTAC-3984
Peptide Molecular Mass: 1,477.7525
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers