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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CS N/A
Sequence Length (AA) Molecular Weight (Da)
466 51712
Protein Name
Citrate synthase, mitochondrial
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALLTAAARL LGTKNASCLV LAARHASASS TNLKDILADL IPKEQARIKT
60 70 80 90 100
FRQQHGKTVV GQITVDMMYG GMRGMKGLVY ETSVLDPDEG IRFRGFSIPE
110 120 130 140 150
CQKLLPKAKG GEEPLPEGLF WLLVTGHIPT EEQVSWLSKE WAKRAALPSH
160 170 180 190 200
VVTMLDNFPT NLHPMSQLSA AVTALNSESN FARAYAQGIS RTKYWELIYE
210 220 230 240 250
DSMDLIAKLP CVAAKIYRNL YREGSGIGAI DSNLDWSHNF TNMLGYTDHQ
260 270 280 290 300
FTELTRLYLT IHSDHEGGNV SAHTSHLVGS ALSDPYLSFA AAMNGLAGPL
310 320 330 340 350
HGLANQEVLV WLTQLQKEVG KDVSDEKLRD YIWNTLNSGR VVPGYGHAVL
360 370 380 390 400
RKTDPRYTCQ REFALKHLPN DPMFKLVAQL YKIVPNVLLE QGKAKNPWPN
410 420 430 440 450
VDAHSGVLLQ YYGMTEMNYY TVLFGVSRAL GVLAQLIWSR ALGFPLERPK
460 466
SMSTEGLMKF VDSKSG

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2721 Collapse assay details

Data source: Panorama

Official Gene Symbol
CS
Peptide Sequence
GLVYETSVLDPDEGIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
77
Peptide End
92
CPTAC ID
CPTAC-2721
Peptide Molecular Mass
1,761.8785
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher Q-Exactive
Internal Standard
13 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 um x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 7.2 3.4 5.4 7.5 3.6 9 10.4 5 10.5 15 15 15
y8 (1+) 5.8 3.4 3.5 6.9 3.4 7.5 9 4.8 8.3 15 15 15
y3 (1+) 6.1 4.4 6.8 7 5.9 8.2 9.3 7.4 10.7 15 15 15
sum 5.7 2.7 4.4 6.2 2.7 7.7 8.4 3.8 8.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2723 Collapse assay details

Data source: Panorama

Official Gene Symbol
CS
Peptide Sequence
IVPNVLLEQGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
383
Peptide End
393
CPTAC ID
CPTAC-2723
Peptide Molecular Mass
1,208.7129
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher Q-Exactive
Internal Standard
13 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 um x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 6.5 6 5.2 6.7 4.4 5.5 9.3 7.4 7.6 15 15 15
y4 (1+) 6.1 7.4 3.9 6.3 6.3 4.7 8.8 9.7 6.1 15 15 15
y3 (1+) 9.6 5.8 6 8.9 6.5 6.4 13.1 8.7 8.8 15 15 15
sum 6.7 5.9 3.9 5.7 4.7 4.2 8.8 7.5 5.7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2722 Collapse assay details

Data source: Panorama

Official Gene Symbol
CS
Peptide Sequence
VVPGYGHAVLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
341
Peptide End
351
CPTAC ID
CPTAC-2722
Peptide Molecular Mass
1,166.6560
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher Q-Exactive
Internal Standard
13 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 um x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 27.7 7.6 10.6 24.3 7.7 10.4 36.8 10.8 14.8 15 15 15
y6 (1+) 20.1 6.1 10 24.2 6.5 8.3 31.5 8.9 13 15 15 15
y4 (1+) 37.7 8.9 8.9 41.2 8.9 13 55.8 12.6 15.8 15 15 15
sum 24.6 6.5 9 23 5.6 9.2 33.7 8.6 12.9 15 15 15


Additional Resources and Comments