CS, CPTAC-2722 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CS	N/A

Sequence Length (AA)	Molecular Weight (Da)
466	51712

Protein Name
Citrate synthase, mitochondrial

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MALLTAAARL	LGTKNASCLV	LAARHASASS	TNLKDILADL	IPKEQARIKT
60	70	80	90	100
FRQQHGKTVV	GQITVDMMYG	GMRGMKGLVY	ETSVLDPDEG	IRFRGFSIPE
110	120	130	140	150
CQKLLPKAKG	GEEPLPEGLF	WLLVTGHIPT	EEQVSWLSKE	WAKRAALPSH
160	170	180	190	200
VVTMLDNFPT	NLHPMSQLSA	AVTALNSESN	FARAYAQGIS	RTKYWELIYE
210	220	230	240	250
DSMDLIAKLP	CVAAKIYRNL	YREGSGIGAI	DSNLDWSHNF	TNMLGYTDHQ
260	270	280	290	300
FTELTRLYLT	IHSDHEGGNV	SAHTSHLVGS	ALSDPYLSFA	AAMNGLAGPL
310	320	330	340	350
HGLANQEVLV	WLTQLQKEVG	KDVSDEKLRD	YIWNTLNSGR	VVPGYGHAVL
360	370	380	390	400
RKTDPRYTCQ	REFALKHLPN	DPMFKLVAQL	YKIVPNVLLE	QGKAKNPWPN
410	420	430	440	450
VDAHSGVLLQ	YYGMTEMNYY	TVLFGVSRAL	GVLAQLIWSR	ALGFPLERPK
460	466
SMSTEGLMKF	VDSKSG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2721 Collapse assay details

Data source: Panorama

Official Gene Symbol: CS
Peptide Sequence: GLVYETSVLDPDEGIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 77
Peptide End: 92

CPTAC ID: CPTAC-2721
Peptide Molecular Mass: 1,761.8785
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher Q-Exactive
Internal Standard: 13 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 um x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	7.2	3.4	5.4	7.5	3.6	9	10.4	5	10.5	15	15	15
y8 (1+)	5.8	3.4	3.5	6.9	3.4	7.5	9	4.8	8.3	15	15	15
y3 (1+)	6.1	4.4	6.8	7	5.9	8.2	9.3	7.4	10.7	15	15	15
sum	5.7	2.7	4.4	6.2	2.7	7.7	8.4	3.8	8.9	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2723 Collapse assay details

Data source: Panorama

Official Gene Symbol: CS
Peptide Sequence: IVPNVLLEQGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 383
Peptide End: 393

CPTAC ID: CPTAC-2723
Peptide Molecular Mass: 1,208.7129
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend