FBP2, CPTAC-2731 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FBP2	N/A

Sequence Length (AA)	Molecular Weight (Da)
339	36743

Protein Name
Fructose-1,6-bisphosphatase isozyme 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTDRSPFETD	MLTLTRYVME	KGRQAKGTGE	LTQLLNSMLT	AIKAISSAVR
60	70	80	90	100
KAGLAHLYGI	AGSVNVTGDE	VKKLDVLSNS	LVINMVQSSY	STCVLVSEEN
110	120	130	140	150
KDAIITAKEK	RGKYVVCFDP	LDGSSNIDCL	ASIGTIFAIY	RKTSEDEPSE
160	170	180	190	200
KDALQCGRNI	VAAGYALYGS	ATLVALSTGQ	GVDLFMLDPA	LGEFVLVEKD
210	220	230	240	250
VKIKKKGKIY	SLNEGYAKYF	DAATTEYVQK	KKFPEDGSAP	YGARYVGSMV
260	270	280	290	300
ADVHRTLVYG	GIFLYPANQK	SPKGKLRLLY	ECNPVAYIIE	QAGGLATTGT
310	320	330	339
QPVLDVKPEA	IHQRVPLILG	SPEDVQEYLT	CVQKNQAGS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2731 Collapse assay details

Data source: Panorama

Official Gene Symbol: FBP2
Peptide Sequence: YFDAATTEYVQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 219
Peptide End: 230

CPTAC ID: CPTAC-2731
Peptide Molecular Mass: 1,434.6667
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher Q-Exactive
Internal Standard: 13 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 um x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	22.1	5.5	6.2	33.9	6.6	7.1	40.5	8.6	9.4	15	15	15
y5 (1+)	19.6	21.8	11	23.7	21	15.6	30.8	30.3	19.1	13	15	15
y3 (1+)	23	14.6	4.4	41	16.3	5.5	47	21.9	7	15	15	15
sum	19.2	9.7	4.8	33.8	10.7	5.9	38.9	14.4	7.6	15	15	15

FBP2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2731 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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FBP2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2731 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments