FH, CPTAC-2732 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FH	N/A

Sequence Length (AA)	Molecular Weight (Da)
510	54637

Protein Name
Fumarate hydratase, mitochondrial

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MYRALRLLAR	SRPLVRAPAA	ALASAPGLGG	AAVPSFWPPN	AARMASQNSF
60	70	80	90	100
RIEYDTFGEL	KVPNDKYYGA	QTVRSTMNFK	IGGVTERMPT	PVIKAFGILK
110	120	130	140	150
RAAAEVNQDY	GLDPKIANAI	MKAADEVAEG	KLNDHFPLVV	WQTGSGTQTN
160	170	180	190	200
MNVNEVISNR	AIEMLGGELG	SKIPVHPNDH	VNKSQSSNDT	FPTAMHIAAA
210	220	230	240	250
IEVHEVLLPG	LQKLHDALDA	KSKEFAQIIK	IGRTHTQDAV	PLTLGQEFSG
260	270	280	290	300
YVQQVKYAMT	RIKAAMPRIY	ELAAGGTAVG	TGLNTRIGFA	EKVAAKVAAL
310	320	330	340	350
TGLPFVTAPN	KFEALAAHDA	LVELSGAMNT	TACSLMKIAN	DIRFLGSGPR
360	370	380	390	400
SGLGELILPE	NEPGSSIMPG	KVNPTQCEAM	TMVAAQVMGN	HVAVTVGGSN
410	420	430	440	450
GHFELNVFKP	MMIKNVLHSA	RLLGDASVSF	TENCVVGIQA	NTERINKLMN
460	470	480	490	500
ESLMLVTALN	PHIGYDKAAK	IAKTAHKNGS	TLKETAIELG	YLTAEQFDEW
510
VKPKDMLGPK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3579 Collapse assay details

Data source: Panorama

Official Gene Symbol: Fh
Peptide Sequence: YYGAQTVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 64
Peptide End: 71

CPTAC ID: non-CPTAC-3579
Peptide Molecular Mass: 956.4716
Species: Mus musculus (Mouse)
Assay Type: Direct PRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	17.5	7.9	10.6	15.8	11.7	9.6	23.6	14.1	14.3	15	15	15
y7 (2+)	86.5	22.3	17.6	86	21.4	17.8	122	30.9	25	15	15	15
y4 (1+)	30.5	11.3	16.6	30.4	11.1	15.3	43.1	15.8	22.6	15	15	15
y6 (1+)	6.2	7	5.2	11.2	10.8	5.9	12.8	12.9	7.9	15	15	15
y7 (1+)	37.9	12.4	6	46.9	15.3	9.6	60.3	19.7	11.3	15	15	15
sum	7.8	5.6	4.1	11	10	4.4	13.5	11.5	6	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2732 Collapse assay details

Data source: Panorama

Official Gene Symbol: FH
Peptide Sequence: IYELAAGGTAVGTGLNTR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 269
Peptide End: 286

CPTAC ID: CPTAC-2732
Peptide Molecular Mass: 1,762.9214
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend