G6PD, CPTAC-2734 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
G6PD	N/A

Sequence Length (AA)	Molecular Weight (Da)
515	59257

Protein Name
Glucose-6-phosphate 1-dehydrogenase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAEQVALSRT	QVCGILREEL	FQGDAFHQSD	THIFIIMGAS	GDLAKKKIYP
60	70	80	90	100
TIWWLFRDGL	LPENTFIVGY	ARSRLTVADI	RKQSEPFFKA	TPEEKLKLED
110	120	130	140	150
FFARNSYVAG	QYDDAASYQR	LNSHMNALHL	GSQANRLFYL	ALPPTVYEAV
160	170	180	190	200
TKNIHESCMS	QIGWNRIIVE	KPFGRDLQSS	DRLSNHISSL	FREDQIYRID
210	220	230	240	250
HYLGKEMVQN	LMVLRFANRI	FGPIWNRDNI	ACVILTFKEP	FGTEGRGGYF
260	270	280	290	300
DEFGIIRDVM	QNHLLQMLCL	VAMEKPASTN	SDDVRDEKVK	VLKCISEVQA
310	320	330	340	350
NNVVLGQYVG	NPDGEGEATK	GYLDDPTVPR	GSTTATFAAV	VLYVENERWD
360	370	380	390	400
GVPFILRCGK	ALNERKAEVR	LQFHDVAGDI	FHQQCKRNEL	VIRVQPNEAV
410	420	430	440	450
YTKMMTKKPG	MFFNPEESEL	DLTYGNRYKN	VKLPDAYERL	ILDVFCGSQM
460	470	480	490	500
HFVRSDELRE	AWRIFTPLLH	QIELEKPKPI	PYIYGSRGPT	EADELMKRVG
510	515
FQYEGTYKWV	NPHKL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-205 Collapse assay details

Data source: Panorama

Official Gene Symbol: G6PD
Peptide Sequence: GGYFDEFGIIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 247
Peptide End: 257

CPTAC ID: CPTAC-205
Peptide Molecular Mass: 1,272.6139
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	9.6	8.8	6.7	18.4	12.9	15.6	20.8	15.6	17	15	25	15
y7 (1+)	9.3	6.3	6.2	17	10.4	14.5	19.4	12.2	15.8	15	25	15
y6 (1+)	13.5	6.5	5.6	18.8	9.5	12.6	23.1	11.5	13.8	15	25	15
sum	7.3	6.3	5.9	16.1	10.6	14.3	17.7	12.3	15.5	15	25	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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