GPI, CPTAC-2735 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GPI	N/A

Sequence Length (AA)	Molecular Weight (Da)
558	63147

Protein Name
Glucose-6-phosphate isomerase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAALTRDPQF	QKLQQWYREH	RSELNLRRLF	DANKDRFNHF	SLTLNTNHGH
60	70	80	90	100
ILVDYSKNLV	TEDVMRMLVD	LAKSRGVEAA	RERMFNGEKI	NYTEGRAVLH
110	120	130	140	150
VALRNRSNTP	ILVDGKDVMP	EVNKVLDKMK	SFCQRVRSGD	WKGYTGKTIT
160	170	180	190	200
DVINIGIGGS	DLGPLMVTEA	LKPYSSGGPR	VWYVSNIDGT	HIAKTLAQLN
210	220	230	240	250
PESSLFIIAS	KTFTTQETIT	NAETAKEWFL	QAAKDPSAVA	KHFVALSTNT
260	270	280	290	300
TKVKEFGIDP	QNMFEFWDWV	GGRYSLWSAI	GLSIALHVGF	DNFEQLLSGA
310	320	330	340	350
HWMDQHFRTT	PLEKNAPVLL	ALLGIWYINC	FGCETHAMLP	YDQYLHRFAA
360	370	380	390	400
YFQQGDMESN	GKYITKSGTR	VDHQTGPIVW	GEPGTNGQHA	FYQLIHQGTK
410	420	430	440	450
MIPCDFLIPV	QTQHPIRKGL	HHKILLANFL	AQTEALMRGK	STEEARKELQ
460	470	480	490	500
AAGKSPEDLE	RLLPHKVFEG	NRPTNSIVFT	KLTPFMLGAL	VAMYEHKIFV
510	520	530	540	550
QGIIWDINSF	DQWGVELGKQ	LAKKIEPELD	GSAQVTSHDA	STNGLINFIK
558
QQREARVQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2735 Collapse assay details

Data source: Panorama

Official Gene Symbol: GPI
Peptide Sequence: VWYVSNIDGTHIAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 181
Peptide End: 194

CPTAC ID: CPTAC-2735
Peptide Molecular Mass: 1,601.8202
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher Q-Exactive
Internal Standard: 13 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 um x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	4.6	3.5	6.5	5.2	2.9	7.3	6.9	4.5	9.8	15	15	15
y7 (1+)	6.7	3.3	8	6.8	5.2	8.5	9.5	6.2	11.7	15	15	15
y10 (1+)	5.8	4.3	9.1	7.6	5.7	9.4	9.6	7.1	13.1	15	15	15
sum	4.8	3.3	7.8	5.3	4	8.1	7.2	5.2	11.2	15	15	15

GPI

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2735 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

GPI

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2735 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments