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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

SLC2A12

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SLC2A12 GLUT12, GLUT8
Sequence Length (AA) Molecular Weight (Da)
617 66966
Protein Name
Solute carrier family 2, facilitated glucose transporter member 12
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVPVENTEGP SLLNQKGTAV ETEGSGSRHP PWARGCGMFT FLSSVTAAVS
60 70 80 90 100
GLLVGYELGI ISGALLQIKT LLALSCHEQE MVVSSLVIGA LLASLTGGVL
110 120 130 140 150
IDRYGRRTAI ILSSCLLGLG SLVLILSLSY TVLIVGRIAI GVSISLSSIA
160 170 180 190 200
TCVYIAEIAP QHRRGLLVSL NELMIVIGIL SAYISNYAFA NVFHGWKYMF
210 220 230 240 250
GLVIPLGVLQ AIAMYFLPPS PRFLVMKGQE GAASKVLGRL RALSDTTEEL
260 270 280 290 300
TVIKSSLKDE YQYSFWDLFR SKDNMRTRIM IGLTLVFFVQ ITGQPNILFY
310 320 330 340 350
ASTVLKSVGF QSNEAASLAS TGVGVVKVIS TIPATLLVDH VGSKTFLCIG
360 370 380 390 400
SSVMAASLVT MGIVNLNIHM NFTHICRSHN SINQSLDESV IYGPGNLSTN
410 420 430 440 450
NNTLRDHFKG ISSHSRSSLM PLRNDVDKRG ETTSASLLNA GLSHTEYQIV
460 470 480 490 500
TDPGDVPAFL KWLSLASLLV YVAAFSIGLG PMPWLVLSEI FPGGIRGRAM
510 520 530 540 550
ALTSSMNWGI NLLISLTFLT VTDLIGLPWV CFIYTIMSLA SLLFVVMFIP
560 570 580 590 600
ETKGCSLEQI SMELAKVNYV KNNICFMSHH QEELVPKQPQ KRKPQEQLLE
610 617
CNKLCGRGQS RQLSPET

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2738 Collapse assay details

Data source: Panorama

Official Gene Symbol
SLC2A12
Peptide Sequence
ALSDTTEELTVIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
242
Peptide End
254
CPTAC ID
CPTAC-2738
Peptide Molecular Mass
1,418.7504
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher Q-Exactive
Internal Standard
13 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 um x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 8.1 6.1 8.8 11.7 10 7.5 14.2 11.7 11.6 15 15 15
y6 (1+) 12.2 8.9 11.4 19.5 12.5 10.9 23 15.3 15.8 15 15 15
b4 (1+) 55.6 18.2 21.7 75.3 20.6 28.1 93.6 27.5 35.5 15 15 15
sum 5.6 4.5 7.1 10.2 9.4 7.6 11.6 10.4 10.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
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