IDH3A, CPTAC-2739 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
IDH3A	N/A

Sequence Length (AA)	Molecular Weight (Da)
366	39592

Protein Name
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGPAWISKV	SRLLGAFHNP	KQVTRGFTGG	VQTVTLIPGD	GIGPEISAAV
60	70	80	90	100
MKIFDAAKAP	IQWEERNVTA	IQGPGGKWMI	PSEAKESMDK	NKMGLKGPLK
110	120	130	140	150
TPIAAGHPSM	NLLLRKTFDL	YANVRPCVSI	EGYKTPYTDV	NIVTIRENTE
160	170	180	190	200
GEYSGIEHVI	VDGVVQSIKL	ITEGASKRIA	EFAFEYARNN	HRSNVTAVHK
210	220	230	240	250
ANIMRMSDGL	FLQKCREVAE	SCKDIKFNEM	YLDTVCLNMV	QDPSQFDVLV
260	270	280	290	300
MPNLYGDILS	DLCAGLIGGL	GVTPSGNIGA	NGVAIFESVH	GTAPDIAGKD
310	320	330	340	350
MANPTALLLS	AVMMLRHMGL	FDHAARIEAA	CFATIKDGKS	LTKDLGGNAK
360	366
CSDFTEEICR	RVKDLD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3649 Collapse assay details

Data source: Panorama

Official Gene Symbol: Idh3a
Peptide Sequence: IAEFAFEYAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 179
Peptide End: 188

CPTAC ID: non-CPTAC-3649
Peptide Molecular Mass: 1,215.5924
Species: Mus musculus (Mouse)
Assay Type: Direct PRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	5.3	4.2	2.8	7.9	4.7	3.9	9.5	6.3	4.8	15	15	15
y6 (1+)	9.6	4.3	2.5	12.2	5.3	3.4	15.5	6.8	4.2	15	15	15
y7 (1+)	5.5	3.2	1.4	10.2	5	4.1	11.6	5.9	4.3	15	15	15
y8 (1+)	6.7	2.4	2.9	13.9	4.4	3.6	15.4	5	4.6	15	15	15
b3 (1+)	6	5	4.4	12	5.6	6.1	13.4	7.5	7.5	15	15	15
sum	3	1.3	1	9.7	3.5	2.7	10.2	3.7	2.9	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2739 Collapse assay details

Data source: Panorama

Official Gene Symbol: IDH3A
Peptide Sequence: APIQWEER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 59
Peptide End: 66

CPTAC ID: CPTAC-2739
Peptide Molecular Mass: 1,027.5087
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend