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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Idh3a N/A
Sequence Length (AA) Molecular Weight (Da)
366 39639
Protein Name
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGSAWVSKV SRLLGAFHNT KQVTRGFAGG VQTVTLIPGD GIGPEISASV
60 70 80 90 100
MKIFDAAKAP IQWEERNVTA IQGPGGKWMI PPEAKESMDK NKMGLKGPLK
110 120 130 140 150
TPIAAGHPSM NLLLRKTFDL YANVRPCVSI EGYKTPYTDV NIVTIRENTE
160 170 180 190 200
GEYSGIEHVI VDGVVQSIKL ITEEASKRIA EFAFEYARNN HRSNVTAVHK
210 220 230 240 250
ANIMRMSDGL FLQKCREVAE NCKDIKFNEM YLDTVCLNMV QDPSQFDVLV
260 270 280 290 300
MPNLYGDILS DLCAGLIGGL GVTPSGNIGA NGVAIFESVH GTAPDIAGKD
310 320 330 340 350
MANPTALLLS AVMMLRHMGL FDHAAKIEAA CFATIKDGKS LTKDLGGNAK
360 366
CSDFTEEICR RVKDLD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3649 Collapse assay details

Data source: Panorama

Official Gene Symbol
Idh3a
Peptide Sequence
IAEFAFEYAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
179
Peptide End
188
CPTAC ID
non-CPTAC-3649
Peptide Molecular Mass
1,215.5924
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.3 4.2 2.8 7.9 4.7 3.9 9.5 6.3 4.8 15 15 15
y6 (1+) 9.6 4.3 2.5 12.2 5.3 3.4 15.5 6.8 4.2 15 15 15
y7 (1+) 5.5 3.2 1.4 10.2 5 4.1 11.6 5.9 4.3 15 15 15
y8 (1+) 6.7 2.4 2.9 13.9 4.4 3.6 15.4 5 4.6 15 15 15
b3 (1+) 6 5 4.4 12 5.6 6.1 13.4 7.5 7.5 15 15 15
sum 3 1.3 1 9.7 3.5 2.7 10.2 3.7 2.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2739 Collapse assay details

Data source: Panorama

Official Gene Symbol
IDH3A
Peptide Sequence
APIQWEER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
59
Peptide End
66
CPTAC ID
CPTAC-2739
Peptide Molecular Mass
1,027.5087
Species
Homo Sapiens
Assay Type
Direct MRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher Q-Exactive
Internal Standard
13 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 um x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 23 7.9 7.9 27.7 9.3 6.9 36 12.2 10.5 15 15 15
y4 (1+) 5.1 3.7 7.3 5.9 2.9 6.2 7.8 4.7 9.6 15 15 15
y5 (1+) 6.1 3.1 5.7 7.4 3.2 5.6 9.6 4.5 8 15 15 15
sum 5.1 3.2 6 5.9 3.2 5.3 7.8 4.5 8 15 15 15


Additional Resources and Comments