CCNB2, CPTAC-2799 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CCNB2	N/A

Sequence Length (AA)	Molecular Weight (Da)
398	45282

Protein Name
G2/mitotic-specific cyclin-B2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MALLRRPTVS	SDLENIDTGV	NSKVKSHVTI	RRTVLEEIGN	RVTTRAAQVA
60	70	80	90	100
KKAQNTKVPV	QPTKTTNVNK	QLKPTASVKP	VQMEKLAPKG	PSPTPEDVSM
110	120	130	140	150
KEENLCQAFS	DALLCKIEDI	DNEDWENPQL	CSDYVKDIYQ	YLRQLEVLQS
160	170	180	190	200
INPHFLDGRD	INGRMRAILV	DWLVQVHSKF	RLLQETLYMC	VGIMDRFLQV
210	220	230	240	250
QPVSRKKLQL	VGITALLLAS	KYEEMFSPNI	EDFVYITDNA	YTSSQIREME
260	270	280	290	300
TLILKELKFE	LGRPLPLHFL	RRASKAGEVD	VEQHTLAKYL	MELTLIDYDM
310	320	330	340	350
VHYHPSKVAA	AASCLSQKVL	GQGKWNLKQQ	YYTGYTENEV	LEVMQHMAKN
360	370	380	390	398
VVKVNENLTK	FIAIKNKYAS	SKLLKISMIP	QLNSKAVKDL	ASPLIGRS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2800 Collapse assay details

Data source: Panorama

Official Gene Symbol: CCNB2
Peptide Sequence: AGEVDVEQHTLAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 276
Peptide End: 288

CPTAC ID: CPTAC-2800
Peptide Molecular Mass: 1,395.6994
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	4.1	4.5	4.5	6.1	5.2	4.8	7.3	6.9	6.6	15	15	15
y5 (1+)	4.1	4.5	5.6	4.5	5.6	6.8	6.1	7.2	8.8	15	15	15
y4 (1+)	6.2	5.6	4.2	7.7	6.2	6.2	9.9	8.4	7.5	15	15	15
sum	3	4.1	3.7	4.8	4.5	4.8	5.7	6.1	6.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2799 Collapse assay details

Data source: Panorama

Official Gene Symbol: CCNB2
Peptide Sequence: DLASPLIGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 389
Peptide End: 397

CPTAC ID: CPTAC-2799
Peptide Molecular Mass: 940.5342
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend