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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CCNB1 CCNB
Sequence Length (AA) Molecular Weight (Da)
433 48337
Protein Name
G2/mitotic-specific cyclin-B1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALRVTRNSK INAENKAKIN MAGAKRVPTA PAATSKPGLR PRTALGDIGN
60 70 80 90 100
KVSEQLQAKM PMKKEAKPSA TGKVIDKKLP KPLEKVPMLV PVPVSEPVPE
110 120 130 140 150
PEPEPEPEPV KEEKLSPEPI LVDTASPSPM ETSGCAPAEE DLCQAFSDVI
160 170 180 190 200
LAVNDVDAED GADPNLCSEY VKDIYAYLRQ LEEEQAVRPK YLLGREVTGN
210 220 230 240 250
MRAILIDWLV QVQMKFRLLQ ETMYMTVSII DRFMQNNCVP KKMLQLVGVT
260 270 280 290 300
AMFIASKYEE MYPPEIGDFA FVTDNTYTKH QIRQMEMKIL RALNFGLGRP
310 320 330 340 350
LPLHFLRRAS KIGEVDVEQH TLAKYLMELT MLDYDMVHFP PSQIAAGAFC
360 370 380 390 400
LALKILDNGE WTPTLQHYLS YTEESLLPVM QHLAKNVVMV NQGLTKHMTV
410 420 430 433
KNKYATSKHA KISTLPQLNS ALVQDLAKAV AKV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1236 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCNB1
Peptide Sequence
TALGDIGNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
43
Peptide End
51
CPTAC ID
CPTAC-1236
Peptide Molecular Mass
887.4712
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 31.8 10.7 9.5 42.1 10.7 10.9 52.8 15.1 14.5 15 15 15
y6 (1+) 23.6 7.6 8.5 27.1 9.7 8.1 35.9 12.3 11.7 15 15 15
y7 (1+) 8.5 7.1 4.9 19.3 9.6 8 21.1 11.9 9.4 15 15 15
sum 8.4 6.5 5.5 18 8.5 6.5 19.9 10.7 8.5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2797 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCNB1
Peptide Sequence
ISTLPQLNSALVQDLAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
412
Peptide End
428
CPTAC ID
CPTAC-2797
Peptide Molecular Mass
1,810.0200
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (1+) 7.1 7.1 9.3 8.3 12.7 9.1 10.9 14.5 13 15 15 15
y10 (1+) 7.5 7.5 10.4 8.7 12.6 10.5 11.5 14.7 14.8 15 15 15
y5 (1+) 7 7.2 11.7 8.1 12.2 12.3 10.7 14.2 17 15 15 15
sum 7.1 7.2 9.8 8.3 12.6 9.8 10.9 14.5 13.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2798 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCNB1
Peptide Sequence
TALGDIGNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
43
Peptide End
51
CPTAC ID
CPTAC-2798
Peptide Molecular Mass
887.4712
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 2.9 4.8 5.1 2.9 5.8 5.2 4.1 7.5 7.3 15 15 15
y5 (1+) 6.1 4.5 5.5 6.3 8.4 6 8.8 9.5 8.1 15 15 15
y3 (1+) 5.5 4.1 3.3 4.8 5.7 3.8 7.3 7 5 15 15 15
sum 3 4.3 4.1 2 5.5 4.2 3.6 7 5.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2795 Collapse assay details

Data source: Panorama

Official Gene Symbol
CCNB1
Peptide Sequence
VSEQLQAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
52
Peptide End
59
CPTAC ID
CPTAC-2795
Peptide Molecular Mass
901.4869
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 9.7 9.9 4 11.8 8.9 4 15.3 13.3 5.7 15 15 15
y4 (1+) 8 10.6 3.5 11.1 13 2.2 13.7 16.8 4.1 15 15 15
y3 (1+) 12.7 9.8 3.7 16.4 9.7 5.4 20.7 13.8 6.5 15 15 15
sum 7.6 9.4 3 10 10.2 2.5 12.6 13.9 3.9 15 15 15


Additional Resources and Comments